Overview
- External ontology
- UTEX:desmid-medium (source link unavailable) — Desmid Medium
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- UTEX prepares Desmid Medium as a Pringsheim desmidiacean modification with Soilwater: GR+ Medium, magnesium sulfate, phosphate, and nitrate.
Composition
flowchart LR
CultureMech_000162["desmid_medium"]
ing0_dH2O["dH2O"]
CultureMech_000162 --> ing0_dH2O
CHEBI_15377["CHEBI:15377"]
ing0_dH2O -.-> CHEBI_15377
ing1_Soilwater__GR__Medium["Soilwater: GR+ Medium"]
CultureMech_000162 --> ing1_Soilwater__GR__Medium
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000162 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_K2HPO4["K2HPO4"]
CultureMech_000162 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_KNO3["KNO3"]
CultureMech_000162 --> ing4_KNO3
CHEBI_63043["CHEBI:63043"]
ing4_KNO3 -.-> CHEBI_63043
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
dH2O
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
UTEX starts the 1 L Desmid Medium recipe with approximately 900 mL dH2O. |
| 2 | Soilwater: GR+ Medium | 50 ML_PER_L | UTEX lists 50 mL prepared Soilwater: GR+ Medium per liter. | |
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
Sigma 230391
|
0.04 MILLIMOLAR |
CHEBI:31795
magnesium sulfate heptahydrate
|
UTEX adds 10 mL/L of a 0.2 g/200 mL dH2O MgSO4 x 7 H2O stock. |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
Sigma P 3786
|
0.057 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
UTEX adds 10 mL/L of a 0.2 g/200 mL dH2O K2HPO4 stock. |
| 5 |
KNO3
K.NO3 101.102 g/mol
Baker 3190
|
0.99 MILLIMOLAR |
CHEBI:63043
potassium nitrate
|
UTEX adds 10 mL/L of a 2 g/200 mL dH2O KNO3 stock. |
Preparation
- To approximately 900 mL dH2O, add components in the listed order while stirring continuously.
- Bring the total volume to 1 L with dH2O.
- Cover and autoclave the medium.
- Store at refrigerator temperature.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | utex-import | Imported from UTEX Culture Collection | Source ID: desmid-medium, URL: https://utex.org/products/desmid-medium |
| 2026-03-13T06:18:53.357756Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000162 |
| 2026-03-17T19:45:04.299302+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Desmid Medium" to "desmid_medium" for consistent matching |
| 2026-05-16T09:02:55.973993+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1 |
| 2026-05-16T09:11:01.172602+00:00 | migrate_preparation_steps.py | MIGRATED_PREPARATION_STEPS | instruction->action+description for 9 step(s) |
| 2026-08-30T00:58:30.763377+00:00 | repair_utex_ingredients.py | REPAIRED_UTEX_COMPOSITION | Restored 4 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779. |
| 2026-09-06T00:00:00-07:00 | repair_utex_simple_score35.py | RESOLVED_UTEX_SIMPLE_SCORE35 | UTEX prepares Desmid Medium as a Pringsheim desmidiacean modification with Soilwater: GR+ Medium, magnesium sulfate, phosphate, and nitrate. |