Overview
- External ontology
- UTEX:es-over-4-enriched-seawater-medium (source link unavailable) — ES/4 Enriched Seawater Medium
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- UTEX prepares this lower-enrichment seawater variant by adding 5 mL sterile ES Enrichment Solution per liter of Pasteurized Seawater.
Composition
flowchart LR
CultureMech_000165["es_4_enriched_seawater_medium"]
ing0_Pasteurized_Seawater["Pasteurized Seawater"]
CultureMech_000165 --> ing0_Pasteurized_Seawater
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Pasteurized Seawater | 1 L |
ENVO:00002149
sea water
|
Use 1 L pasteurized seawater as the absolute input before adding enrichment. This is not a per-liter finished-medium solvent concentration. |
Preparation
- Aseptically add 5 mL of sterile ES Enrichment Solution per liter of Pasteurized Seawater.
- Store at refrigerator temperature.
Solutions
Enrichment Solution for Seawater Medium
Dose: 0.005 L
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | UTEX brings ES Enrichment Solution to 2 L with dH2O. The stock/carrier total does not quantify the pure-water input; no fixed solvent fraction is asserted. | |
| NaNO3 | 2.35 G_PER_L | Converted from 4.7 g/2 L in the UTEX Enrichment Solution for Seawater Medium recipe. |
| beta-Glycerol phosphate disodium salt pentahydrate | 0.49 G_PER_L | Converted from 0.98 g/2 L in the UTEX Enrichment Solution for Seawater Medium recipe. Source CAS 13408-09-8 denotes the disodium beta-glycerophosphate pentahydrate, not sn-glycerol 3-phosphate. No exact replacement ontology identifier is asserted. |
| HEPES buffer | 3.25 G_PER_L | Converted from 6.5 g/2 L in the UTEX Enrichment Solution for Seawater Medium recipe. |
UTEX adds 5 mL sterile ES Enrichment Solution per liter of seawater. The recorded liter amount is the absolute enrichment aliquot added to the separate 1 L seawater input, not a dose per measured final mixture volume. The five nested stocks belong to enrichment preparation, not directly to the finished medium.
ES Fe Solution
Dose: 162.5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | UTEX brings the ES Fe Solution stock to 2 L with dH2O. The stock/carrier total does not quantify the pure-water input; no fixed solvent fraction is asserted. | |
| Fe(NH4)2(SO4)2 x 6 H2O | 0.7 G_PER_L | Converted from 1.4 g/2 L in the UTEX ES Fe Solution recipe. Retained for the explicitly named ferrous ammonium sulfate hexahydrate. The source formula/catalog discrepancy remains unresolved; no catalog-driven replacement or molarity is inferred. |
| Na2EDTA x 2 H2O | 0.6 G_PER_L | Converted from 1.2 g/2 L in the UTEX ES Fe Solution recipe. |
UTEX adds 325 mL ES Fe Solution per 2 L ES Enrichment Solution.
P-II Metal Solution
Dose: 162.5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | UTEX brings P-II Metal Solution to 100 mL with dH2O. The stock/carrier total does not quantify the pure-water input; no fixed solvent fraction is asserted. | |
| Na2EDTA x 2 H2O | 1 G_PER_L | Converted from 0.1 g/100 mL in the UTEX P-II Metal Solution recipe. |
| H3BO3 | 1.14 G_PER_L | Converted from 0.114 g/100 mL in the UTEX P-II Metal Solution recipe. |
| FeCl3 x 6 H2O | 0.049 G_PER_L | Converted from 4.9 mg/100 mL in the UTEX P-II Metal Solution recipe. |
| MnSO4 x H2O | 0.164 G_PER_L | Converted from 16.4 mg/100 mL in the UTEX P-II Metal Solution recipe. |
| ZnSO4 x 7 H2O | 0.022 G_PER_L | Converted from 2.2 mg/100 mL in the UTEX P-II Metal Solution recipe. |
| CoCl2 x 6 H2O | 0.0048 G_PER_L | Converted from 0.48 mg/100 mL in the UTEX P-II Metal Solution recipe. |
UTEX adds 325 mL P-II Metal Solution per 2 L ES Enrichment Solution.
Vitamin B12
Dose: 1.5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | UTEX prepares the Vitamin B12 stock in 200 mL HEPES buffer. The stock/carrier total does not quantify the pure-water input; no fixed solvent fraction is asserted. | |
| HEPES buffer pH 7.8 | 12 G_PER_L | Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock. |
| Cyanocobalamin | 0.135 G_PER_L | Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock. The supplied form is cyanocobalamin; the stock mass concentration is unchanged. |
UTEX adds 3 mL Vitamin B12 stock per 2 L ES Enrichment Solution.
Biotin Vitamin Solution
Dose: 1.5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | UTEX prepares the biotin stock in 200 mL HEPES buffer. The stock/carrier total does not quantify the pure-water input; no fixed solvent fraction is asserted. | |
| HEPES buffer pH 7.8 | 12 G_PER_L | Converted from 2.4 g/200 mL in the UTEX biotin stock. |
| Biotin | 0.025 G_PER_L | Converted from 0.005 g/200 mL in the UTEX biotin stock. |
UTEX adds 3 mL biotin stock per 2 L ES Enrichment Solution.
Thiamine Vitamin Solution
Dose: 1.5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | UTEX prepares the thiamine stock in 200 mL HEPES buffer. The stock/carrier total does not quantify the pure-water input; no fixed solvent fraction is asserted. | |
| HEPES buffer pH 7.8 | 12 G_PER_L | Converted from 2.4 g/200 mL in the UTEX thiamine stock. |
| Thiamine hydrochloride | 0.335 G_PER_L | Converted from 0.067 g/200 mL in the UTEX thiamine stock. The supplied form is thiamine hydrochloride; the stock mass concentration is unchanged. |
UTEX adds 3 mL thiamine stock per 2 L ES Enrichment Solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | utex-import | Imported from UTEX Culture Collection | Source ID: es-over-4-enriched-seawater-medium, URL: https://utex.org/products/es-over-4-enriched-seawater-medium |
| 2026-03-13T06:18:53.371220Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000165 |
| 2026-03-17T19:45:04.311249+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ES/4 Enriched Seawater Medium" to "es_4_enriched_seawater_medium" for consistent matching |
| 2026-05-16T09:02:55.986985+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1 |
| 2026-05-16T09:11:01.187825+00:00 | migrate_preparation_steps.py | MIGRATED_PREPARATION_STEPS | instruction->action+description for 9 step(s) |
| 2026-08-30T00:58:30.787209+00:00 | repair_utex_ingredients.py | REPAIRED_UTEX_COMPOSITION | Restored 2 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779. |
| 2026-09-06T00:00:00-07:00 | repair_utex_algal_blends.py | RESOLVED_UTEX_ALGAL_BLEND_GRAPH | UTEX prepares this lower-enrichment seawater variant by adding 5 mL sterile ES Enrichment Solution per liter of Pasteurized Seawater. |
| 2026-10-10T01:47:47.012900+00:00 | codex | CURATED_UTEX_ES_NESTED_STOCKS_AND_INGREDIENT_EVIDENCE | Added 32 claim-linked evidence entries and added or enriched 13 bibliography entries in this record. Related formulation/stock limitations for this review batch: The parent HTML total-volume heading conflicts with the per-liter-of-seawater procedure/PDF. Expanded recipes retain absolute liquid inputs; no measured final volume or final constituent concentrations are inferred. The enrichment source lists vitamin doses but omits a later vitamin-addition instruction; no missing step is invented. ES Fe formula and Sigma F1513 catalog identity remain unreconciled. Only the source-named ferrous-ammonium-sulfate stock mass is supported; no catalog-based replacement or molar conversion is made. The P-II source permits a cobalt sulfate alternative without an exact replacement mass/hydrate. Only the listed cobalt chloride hexahydrate amount is supported. No exact glycerophosphate-pentahydrate ontology identifier was established. Unsupported sn-glycerol-3-phosphate assertions are removed; the immutable MIM index remains unchanged. Generic lighting, temperature, application and growth claims remain outside this ingredient review; no taxa-media associations are added. The 2/3 PDF copyright notice does not establish a publication year for the live HTML. Supplier pages inspected by the web tool are not full downloaded captures. |