erdschreibers_medium

CultureMech:000167 algae COMPLEX LIQUID
Original name: Erdschreiber's Medium

Overview

External ontology
UTEX:erdschreibers-medium (source link unavailable) — Erdschreiber's Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares Erdschreiber's Medium from pasteurized filtered seawater at 30 ppt supplemented with nitrate, phosphate, P-IV Metal Solution, Soilwater: GR+ Medium, and Vitamin B12.

Composition

flowchart LR
    CultureMech_000167["erdschreibers_medium"]
    ing0_Pasteurized_Seawater["Pasteurized Seawater"]
    CultureMech_000167 --> ing0_Pasteurized_Seawater
    ing1_NaNO3["NaNO3"]
    CultureMech_000167 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_Na2HPO4_x_7_H2O["Na2HPO4 x 7 H2O"]
    CultureMech_000167 --> ing2_Na2HPO4_x_7_H2O
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4_x_7_H2O -.-> CHEBI_34683

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Pasteurized Seawater 1000 ML_PER_L ENVO:00002149
sea water
 UTEX starts the 3 L recipe with pasteurized filtered seawater at 30 ppt.
2 NaNO3
NO3.Na 84.994 g/mol
Fisher BP360-500
2.3 MILLIMOLAR CHEBI:63005
sodium nitrate
 UTEX adds 10 mL of 0.7 M NaNO3 stock per 3 L medium.
3 Na2HPO4 x 7 H2O
HO4P.2Na 141.958 g/mol
Sigma S-9390
0.067 MILLIMOLAR CHEBI:34683
disodium hydrogen phosphate heptahydrate
 UTEX adds 10 mL of 0.02 M Na2HPO4 x 7 H2O stock per 3 L medium.

Preparation

  1. Aseptically add sterile nitrate, phosphate, P-IV Metal Solution, Soilwater: GR+ Medium, and Vitamin B12 stocks to 3 L of pasteurized filtered seawater at 30 ppt.
  2. Swirl to mix and store at refrigerator temperature.

Solutions

P-IV Metal Solution

Dose: 12 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings P-IV Metal Solution to 1 L with dH2O.
Na2EDTA x 2 H2O 0.75 G_PER_L UTEX lists 0.75 g/L Na2EDTA x 2 H2O, 2 mM final, and dissolves it fully before the other P-IV components.
FeCl3 x 6 H2O 0.097 G_PER_L UTEX lists 0.097 g/L FeCl3 x 6 H2O, for 0.36 mM final.
MnCl2 x 4 H2O 0.041 G_PER_L UTEX lists 0.041 g/L MnCl2 x 4 H2O, for 0.21 mM final.
ZnCl2 0.005 G_PER_L UTEX lists 0.005 g/L ZnCl2, for 0.037 mM final.
CoCl2 x 6 H2O 0.002 G_PER_L UTEX lists 0.002 g/L CoCl2 x 6 H2O, for 0.0084 mM final.
Na2MoO4 x 2 H2O 0.004 G_PER_L UTEX lists 0.004 g/L Na2MoO4 x 2 H2O, for 0.017 mM final.
UTEX adds 12 mL P-IV Metal Solution per liter.

Soilwater: GR+ Medium

Dose: 50 ML_PER_L

CultureMech:000225

UTEX lists 50 mL prepared Soilwater: GR+ Medium per liter.

Vitamin B12

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the Vitamin B12 stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock.
Vitamin B12 0.135 G_PER_L Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock.
UTEX adds 1 mL Vitamin B12 stock per liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: erdschreibers-medium, URL: https://utex.org/products/erdschreibers-medium
2026-03-13T06:18:53.380556Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000167
2026-03-17T19:45:04.319543+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Erdschreiber's Medium" to "erdschreibers_medium" for consistent matching
2026-05-16T09:02:56.532280+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.652934+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:31.600315+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 6 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_algal_blends.py LINKED_UTEX_ALGAL_BLEND_CHILDREN Added or refreshed reciprocal parent-child link for algae/1_2_Erdschreiber_Medium.yaml.
2026-09-06T00:00:00-07:00 repair_utex_algal_blends.py LINKED_UTEX_ALGAL_BLEND_CHILDREN Added or refreshed reciprocal parent-child link for algae/1_4_erdschreibers_medium.yaml.
2026-09-06T00:00:00-07:00 repair_utex_algal_blends.py LINKED_UTEX_ALGAL_BLEND_CHILDREN Added or refreshed reciprocal parent-child link for algae/20_allen_80_erdschreiber_1_4_medium.yaml.
2026-09-06T00:00:00-07:00 repair_utex_bristol_bold_score35.py RESOLVED_UTEX_BRISTOL_BOLD_SCORE35 UTEX prepares Erdschreiber's Medium from pasteurized filtered seawater at 30 ppt supplemented with nitrate, phosphate, P-IV Metal Solution, Soilwater: GR+ Medium, and Vitamin B12.
2026-09-06T00:00:00-07:00 repair_utex_bristol_bold_score35.py LINKED_UTEX_BRISTOL_BOLD_VARIANTS Added or refreshed reciprocal parent-child link for algae/2x_erdschreibers_medium.yaml.