j_medium

Overview

External ontology

UTEX:j-medium (source link unavailable) — J Medium

Applications

• Algae cultivation
• Phytoplankton culture
• Microalgae research

pH range

{'min': 8.3, 'max': 8.5}

Notes

UTEX prepares J Medium as an alkaline freshwater Microcystis recipe at pH 8.3-8.5 with two macro-component stocks, a calcium chloride stock, and G9 trace metals. The water quantities at the parent and three stock levels are unquantified starting-water/makeup inputs, not 1000 mL/L pure-water fractions. Printed stock-page molarity annotations are not independently certified as receiving concentrations.

Composition

flowchart LR
    CultureMech_000172["j_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000172 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000172 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158

Ingredients (2)

Preparation

1. Add all J Medium components to approximately 950 mL dH2O while stirring continuously.
2. Bring the final volume to 1 L with dH2O.
3. Cover and autoclave the medium.
4. Store at refrigerator temperature.

Solutions

Target organisms

No target organisms are recorded.

Recipe variants

• Name

  J Medium with 1.25% agar

  Relationship

  SUPPLEMENTED_VARIANT

  Description

  Source-listed agar alternative to the liquid formulation.

  Modifications

  • Add 12.5 g agar per 1 L recipe (12.5 g/L; 1.25% w/v) before covering and autoclaving; do not mix at the agar-addition step.

  Purpose

  Solid agar formulation

  Evidence

  • Reference

    https://utex.org/products/j-medium

    Supports

    SUPPORT

    Snippet

    For 1.25% agar medium add 12.5 g of agar into the flask; do not mix.

    Explanation

    Supports variants[0].modifications only. Separate heading excerpt: "For 1 L Total". Calculation: 12.5 g / 1 L = 12.5 g/L. Do not add agar to the liquid parent. Locator: Directions step 2 bullet, before the cover/autoclave step.

Provenance