mes_volvox_medium (CultureMech:000174)

Overview

External ontology

UTEX:mes-volvox-medium (source link unavailable) — MES-Volvox Medium

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

pH

6.7

Notes

UTEX prepares MES-Volvox Medium at pH 6.7 from dH2O, calcium nitrate, magnesium sulfate, sodium glycerophosphate, potassium chloride, MES, P-IV Metal Solution, ammonium chloride, Vitamin B12, and Biotin Vitamin Solution.

Composition

flowchart LR
    CultureMech_000174["mes_volvox_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000174 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000174 --> ing1_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing1_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000174 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_Na2glycerophosphate_x_5_H2O["Na2glycerophosphate x 5 H2O"]
    CultureMech_000174 --> ing3_Na2glycerophosphate_x_5_H2O
    CHEBI_15978["CHEBI:15978"]
    ing3_Na2glycerophosphate_x_5_H2O -.-> CHEBI_15978
    ing4_KCl["KCl"]
    CultureMech_000174 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_MES["MES"]
    CultureMech_000174 --> ing5_MES
    CHEBI_39005["CHEBI:39005"]
    ing5_MES -.-> CHEBI_39005
    ing6_NH4Cl["NH4Cl"]
    CultureMech_000174 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206

Ingredients (7)

#	Ingredient	Concentration	Ontology	Notes
1	dH2O H2O 18.015 g/mol	1000 ML_PER_L	CHEBI:15377 water	UTEX starts the 1 L MES-Volvox Medium recipe with approximately 950 mL dH2O.
2	Ca(NO3)2 x 4 H2O Ca.4H2O.2NO3 236.146 g/mol	0.5 MILLIMOLAR	CHEBI:86159 calcium nitrate tetrahydrate	UTEX adds 1 mL/L of an 11.8 g/100 mL dH2O stock, for 0.5 mM final.
3	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.16 MILLIMOLAR	CHEBI:31795 magnesium sulfate heptahydrate	UTEX adds 1 mL/L of a 4 g/100 mL dH2O stock, for 0.16 mM final.
4	Na2glycerophosphate x 5 H2O C3H9O6P 172.073 g/mol	0.16 MILLIMOLAR	CHEBI:15978 sn-glycerol 3-phosphate	UTEX adds 1 mL/L of a 5 g/100 mL dH2O stock, for 0.16 mM final.
5	KCl Cl.K 74.551 g/mol	0.67 MILLIMOLAR	CHEBI:32588 potassium chloride	UTEX adds 1 mL/L of a 5 g/100 mL dH2O stock, for 0.67 mM final.
6	MES C6H13NO4S 195.24 g/mol	10 MILLIMOLAR	CHEBI:39005 2-(N-morpholino)ethanesulfonic acid	UTEX lists 1.95 g/L MES, for 10 mM final.
7	NH4Cl Cl.H4N 53.492 g/mol	0.5 MILLIMOLAR	CHEBI:31206 ammonium chloride	UTEX adds 1 mL/L of a 2.67 g/100 mL dH2O stock, for 0.5 mM final.

Preparation

To approximately 950 mL dH2O, add each component except vitamins in the order specified while stirring continuously.
Adjust the medium to pH 6.7.
Bring the total volume to 1 L with dH2O.
Cover and autoclave the medium.
Allow the medium to cool, then add sterile vitamins.
Store at refrigerator temperature.

Solutions

P-IV Metal Solution

Dose: 6 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
dH2O	1000 ML_PER_L	UTEX brings P-IV Metal Solution to 1 L with dH2O.
Na2EDTA x 2 H2O	0.75 G_PER_L	UTEX lists 0.75 g/L Na2EDTA x 2 H2O, 2 mM final, and dissolves it fully before the other P-IV components.
FeCl3 x 6 H2O	0.097 G_PER_L	UTEX lists 0.097 g/L FeCl3 x 6 H2O, for 0.36 mM final.
MnCl2 x 4 H2O	0.041 G_PER_L	UTEX lists 0.041 g/L MnCl2 x 4 H2O, for 0.21 mM final.
ZnCl2	0.005 G_PER_L	UTEX lists 0.005 g/L ZnCl2, for 0.037 mM final.
CoCl2 x 6 H2O	0.002 G_PER_L	UTEX lists 0.002 g/L CoCl2 x 6 H2O, for 0.0084 mM final.
Na2MoO4 x 2 H2O	0.004 G_PER_L	UTEX lists 0.004 g/L Na2MoO4 x 2 H2O, for 0.017 mM final.

UTEX adds 6 mL P-IV Metal Solution per liter.

Vitamin B12

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
dH2O	1000 ML_PER_L	UTEX prepares the Vitamin B12 stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8	12 G_PER_L	Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock.
Vitamin B12	0.135 G_PER_L	Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock.

UTEX adds 1 mL Vitamin B12 stock per liter.

Biotin Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
dH2O	1000 ML_PER_L	UTEX prepares the biotin stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8	12 G_PER_L	Converted from 2.4 g/200 mL in the UTEX biotin stock.
Biotin	0.025 G_PER_L	Converted from 0.005 g/200 mL in the UTEX biotin stock.

UTEX adds 1 mL Biotin Vitamin Solution per liter.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	utex-import	Imported from UTEX Culture Collection	Source ID: mes-volvox-medium, URL: https://utex.org/products/mes-volvox-medium
2026-03-13T06:18:53.419062Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000174
2026-03-17T19:45:04.354140+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MES-Volvox Medium" to "mes_volvox_medium" for consistent matching
2026-05-16T09:02:56.719876+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.772877+00:00	migrate_preparation_steps.py	MIGRATED_PREPARATION_STEPS	instruction->action+description for 9 step(s)
2026-08-30T00:58:31.776748+00:00	repair_utex_ingredients.py	REPAIRED_UTEX_COMPOSITION	Restored 9 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00	repair_utex_volvox_p49_score35.py	RESOLVED_UTEX_VOLVOX_P49_SCORE35	UTEX prepares MES-Volvox Medium at pH 6.7 from dH2O, calcium nitrate, magnesium sulfate, sodium glycerophosphate, potassium chloride, MES, P-IV Metal Solution, ammonium chloride, Vitamin B12, and Biotin Vitamin Solution.