modified_2x_chev_soil_medium

CultureMech:000176 algae COMPLEX LIQUID
Original name: Modified 2X CHEV + Soil Medium

Overview

External ontology
UTEX:modified-2x-chev-plus-soil-medium (source link unavailable) — Modified 2X CHEV + Soil Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares Modified 2X CHEV + Soil Medium tube-by-tube from 1 cc CR1 Soil, 0.5 mg/12 mL MgCO3, 10 mL dH2O, and 2 mL prepared F/2 Medium.

Composition

flowchart LR
    CultureMech_000176["modified_2x_chev_soil_medium"]
    ing0_CR1_Soil["CR1 Soil"]
    CultureMech_000176 --> ing0_CR1_Soil
    ing1_MgCO3["MgCO3"]
    CultureMech_000176 --> ing1_MgCO3
    CHEBI_31793["CHEBI:31793"]
    ing1_MgCO3 -.-> CHEBI_31793
    ing2_dH2O["dH2O"]
    CultureMech_000176 --> ing2_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing2_dH2O -.-> CHEBI_15377

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 CR1 Soil 0.001 L ENVO:00001998
soil
 Source batch input: 1 cc CR1 Soil per tube in a 15 mL vessel. Stored as 0.001 L of input, not a concentration or final-volume claim.
2 MgCO3
CO3.Mg 84.313 g/mol
MCIB CB486
0.5 MILLIMOLAR CHEBI:31793
magnesium carbonate
 UTEX lists 0.5 mg/12 mL in its Amount column and 0.5 mM in its Final Concentration column. Molarity is transcribed directly, not calculated from the mass input.
3 dH2O
H2O 18.015 g/mol
0.01 L CHEBI:15377
water
 Source batch input: 10 mL dH2O per tube in a 15 mL vessel. Stored as 0.01 L of input, not a concentration or final-volume claim.

Preparation

  1. Add CR1 Soil, MgCO3, dH2O, and F/2 Medium in order to 15 mL glass tubes.
  2. Process the soil and liquid mixture within 6 hours; autoclave for 15 minutes for routine maintenance, or pasteurize just above 98 C for 2 hours on three successive days.
  3. Allow tubes to gas-equilibrate for 2 days before use.

Solutions

F/2 Medium

Dose: 0.002 L

CultureMech:000170

Source batch input: 2 mL F/2 Medium per tube in a 15 mL vessel. Stored as 0.002 L of input, not a concentration or final-volume claim.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: modified-2x-chev-plus-soil-medium, URL: https://utex.org/products/modified-2x-chev-plus-soil-medium
2026-03-13T06:18:53.428263Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000176
2026-03-17T19:45:04.362979+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified 2X CHEV + Soil Medium" to "modified_2x_chev_soil_medium" for consistent matching
2026-05-16T09:02:56.744504+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.790482+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:31.820727+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 4 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_cr1_s_modified_2x_score35.py RESOLVED_UTEX_CR1_S_MODIFIED_2X_SCORE35 UTEX prepares Modified 2X CHEV + Soil Medium tube-by-tube from 1 cc CR1 Soil, 0.5 mg/12 mL MgCO3, 10 mL dH2O, and 2 mL prepared F/2 Medium.
2026-10-09T23:38:36.585055+00:00 codex CURATED_UTEX_CHEV_BATCH_INPUTS_AND_SCOPED_EVIDENCE L quantities are absolute input volumes per single-tube preparation, not final concentrations. Vessel capacity and summed liquid inputs do not establish final volume. The three fixed drop counts retain unresolved legacy VARIABLE encodings; their new evidence supports notes only. No drop-volume estimate is introduced. Modified CHEV's 1 mg/12 mL versus 0.5 mM source discrepancy remains unresolved. Its new evidence supports the discrepancy note, not the retained molarity. Referenced F/2 composition, chemical mappings, preparation, growth conditions and relationships are preserved, not newly certified. No new taxa-media associations.