modified_artificial_seawater_medium (CultureMech:000178)

Overview

External ontology

UTEX:modified-artificial-seawater-medium (source link unavailable) — Modified Artificial Seawater Medium

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

pH

8.0

Notes

UTEX prepares Modified Artificial Seawater Medium at pH 8.0 from dH2O, artificial seawater salts, Tricine, NH4Cl, prepared Soilwater: GR+ Medium, P-IV Metal Solution, and three sterile vitamin stocks.

Composition

flowchart LR
    CultureMech_000178["modified_artificial_seawater_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000178 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_000178 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000178 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_000178 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaNO3["NaNO3"]
    CultureMech_000178 --> ing4_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing4_NaNO3 -.-> CHEBI_63005
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000178 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_KH2PO4["KH2PO4"]
    CultureMech_000178 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_Tricine["Tricine"]
    CultureMech_000178 --> ing7_Tricine
    CHEBI_46760["CHEBI:46760"]
    ing7_Tricine -.-> CHEBI_46760
    ing8_NH4Cl["NH4Cl"]
    CultureMech_000178 --> ing8_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing8_NH4Cl -.-> CHEBI_31206

Ingredients (9)

#	Ingredient	Concentration	Ontology	Notes
1	dH2O H2O 18.015 g/mol	1000 ML_PER_L	CHEBI:15377 water	UTEX starts with approximately 850 mL dH2O and brings Modified Artificial Seawater Medium to 1 L with dH2O.
2	NaCl Cl.Na 58.443 g/mol Fisher S271-500	0.51 MOLAR	CHEBI:26710 sodium chloride	UTEX lists 30 g/L NaCl, for 0.51 M final.
3	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol Sigma 230391	9.9 MILLIMOLAR	CHEBI:31795 magnesium sulfate heptahydrate	UTEX adds 10 mL/L of a 24.4 g/100 mL dH2O stock.
4	KCl Cl.K 74.551 g/mol Fisher P 217	8 MILLIMOLAR	CHEBI:32588 potassium chloride	UTEX adds 10 mL/L of a 6 g/100 mL dH2O stock.
5	NaNO3 NO3.Na 84.994 g/mol Fisher BP360-500	11.8 MILLIMOLAR	CHEBI:63005 sodium nitrate	UTEX adds 10 mL/L of a 10 g/100 mL dH2O stock.
6	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol Sigma C-3881	2.04 MILLIMOLAR	CHEBI:86158 calcium chloride dihydrate	UTEX adds 10 mL/L of a 3 g/100 mL dH2O stock, for 2.04 mM final.
7	KH2PO4 H2O4P.K 136.084 g/mol Sigma P 0662	0.37 MILLIMOLAR	CHEBI:63036 potassium dihydrogen phosphate	UTEX adds 10 mL/L of a 0.5 g/100 mL dH2O stock.
8	Tricine Sigma T-5816-256	5.58 MILLIMOLAR	CHEBI:46760 Tricine	UTEX adds 10 mL/L of a 10 g/100 mL dH2O stock.
9	NH4Cl Cl.H4N 53.492 g/mol Fisher A 649-500	0.5 MILLIMOLAR	CHEBI:31206 ammonium chloride	UTEX adds 1 mL/L of a 2.7 g/100 mL dH2O stock.

Preparation

To approximately 850 mL dH2O, add each component except vitamins in the order specified while stirring continuously.
Adjust the medium to pH 8.0.
Bring the total volume to 1 L with dH2O.
Cover and autoclave the medium.
Allow the medium to cool, then add sterile vitamins.
Store at refrigerator temperature.

Solutions

P-IV Metal Solution

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
dH2O	1000 ML_PER_L	UTEX brings P-IV Metal Solution to 1 L with dH2O.
Na2EDTA x 2 H2O	0.75 G_PER_L	UTEX lists 0.75 g/L Na2EDTA x 2 H2O, 2 mM final, and dissolves it fully before the other P-IV components.
FeCl3 x 6 H2O	0.097 G_PER_L	UTEX lists 0.097 g/L FeCl3 x 6 H2O, for 0.36 mM final.
MnCl2 x 4 H2O	0.041 G_PER_L	UTEX lists 0.041 g/L MnCl2 x 4 H2O, for 0.21 mM final.
ZnCl2	0.005 G_PER_L	UTEX lists 0.005 g/L ZnCl2, for 0.037 mM final.
CoCl2 x 6 H2O	0.002 G_PER_L	UTEX lists 0.002 g/L CoCl2 x 6 H2O, for 0.0084 mM final.
Na2MoO4 x 2 H2O	0.004 G_PER_L	UTEX lists 0.004 g/L Na2MoO4 x 2 H2O, for 0.017 mM final.

UTEX adds 1 mL P-IV Metal Solution per liter.

Soilwater: GR+ Medium

Dose: 30 ML_PER_L

CultureMech:000225

UTEX lists 30 mL prepared Soilwater: GR+ Medium per liter.

Vitamin B12

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
dH2O	1000 ML_PER_L	UTEX prepares the Vitamin B12 stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8	12 G_PER_L	Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock.
Vitamin B12	0.135 G_PER_L	Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock.

UTEX adds 1 mL Vitamin B12 stock per liter.

Biotin Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
dH2O	1000 ML_PER_L	UTEX prepares the biotin stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8	12 G_PER_L	Converted from 2.4 g/200 mL in the UTEX biotin stock.
Biotin	0.025 G_PER_L	Converted from 0.005 g/200 mL in the UTEX biotin stock.

UTEX adds 1 mL Biotin Vitamin Solution per liter.

Thiamine Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
dH2O	1000 ML_PER_L	UTEX prepares the thiamine stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8	12 G_PER_L	Converted from 2.4 g/200 mL in the UTEX thiamine stock.
Thiamine	0.335 G_PER_L	Converted from 0.067 g/200 mL in the UTEX thiamine stock.

UTEX adds 1 mL Thiamine Vitamin Solution per liter.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	utex-import	Imported from UTEX Culture Collection	Source ID: modified-artificial-seawater-medium, URL: https://utex.org/products/modified-artificial-seawater-medium
2026-03-13T06:18:53.438935Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000178
2026-03-17T19:45:04.372745+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Modified Artificial Seawater Medium" to "modified_artificial_seawater_medium" for consistent matching
2026-05-16T09:02:56.036821+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.242536+00:00	migrate_preparation_steps.py	MIGRATED_PREPARATION_STEPS	instruction->action+description for 9 step(s)
2026-08-30T00:58:30.862201+00:00	repair_utex_ingredients.py	REPAIRED_UTEX_COMPOSITION	Restored 13 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00	repair_utex_modified_ldm_score35.py	RESOLVED_UTEX_MODIFIED_LDM_SCORE35	UTEX prepares Modified Artificial Seawater Medium at pH 8.0 from dH2O, artificial seawater salts, Tricine, NH4Cl, prepared Soilwater: GR+ Medium, P-IV Metal Solution, and three sterile vitamin stocks.