modified_bolds_3n_medium

CultureMech:000179 algae COMPLEX LIQUID
Original name: Modified Bolds 3N Medium

Overview

External ontology
UTEX:modified-bold-3n-medium (source link unavailable) — Modified Bold 3N Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH
6.2
Notes
UTEX prepares Modified Bold 3N Medium at pH 6.2 with the high-nitrate Bold 3N salts, P-IV Metal Solution, Soilwater: GR+ Medium, and Vitamin B12, Biotin, and Thiamine stocks.

Composition

flowchart LR
    CultureMech_000179["modified_bolds_3n_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000179 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_NaNO3["NaNO3"]
    CultureMech_000179 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000179 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000179 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_K2HPO4["K2HPO4"]
    CultureMech_000179 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_KH2PO4["KH2PO4"]
    CultureMech_000179 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NaCl["NaCl"]
    CultureMech_000179 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts the 1 L Modified Bold 3N Medium recipe with approximately 900 mL dH2O.
2 NaNO3
NO3.Na 84.994 g/mol
Fisher BP360-500
8.82 MILLIMOLAR CHEBI:63005
sodium nitrate
 UTEX adds 30 mL/L of a 10 g/400 mL dH2O stock, for 8.82 mM final.
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
Sigma C-3881
0.17 MILLIMOLAR CHEBI:86158
calcium chloride dihydrate
 UTEX adds 10 mL/L of a 1 g/400 mL dH2O stock, for 0.17 mM final.
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
Sigma 230391
0.3 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 UTEX adds 10 mL/L of a 3 g/400 mL dH2O stock, for 0.3 mM final.
5 K2HPO4
HO4P.2K 174.174 g/mol
Sigma P 3786
0.43 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
 UTEX adds 10 mL/L of a 3 g/400 mL dH2O stock, for 0.43 mM final.
6 KH2PO4
H2O4P.K 136.084 g/mol
Sigma P 0662
1.29 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
 UTEX adds 10 mL/L of a 7 g/400 mL dH2O stock, for 1.29 mM final.
7 NaCl
Cl.Na 58.443 g/mol
Fisher S271-500
0.43 MILLIMOLAR CHEBI:26710
sodium chloride
 UTEX adds 10 mL/L of a 1 g/400 mL dH2O stock, for 0.43 mM final.

Preparation

  1. To approximately 900 mL dH2O, add the Bristol mineral stocks and P-IV Metal Solution and Soilwater: GR+ Medium.
  2. Adjust the medium to pH 6.2.
  3. Bring the total volume to 1 L with dH2O.
  4. Cover and autoclave the medium.
  5. Add sterile vitamin stocks after the medium cools.
  6. Store at refrigerator temperature.

Solutions

P-IV Metal Solution

Dose: 6 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings P-IV Metal Solution to 1 L with dH2O.
Na2EDTA x 2 H2O 0.75 G_PER_L UTEX lists 0.75 g/L Na2EDTA x 2 H2O, 2 mM final, and dissolves it fully before the other P-IV components.
FeCl3 x 6 H2O 0.097 G_PER_L UTEX lists 0.097 g/L FeCl3 x 6 H2O, for 0.36 mM final.
MnCl2 x 4 H2O 0.041 G_PER_L UTEX lists 0.041 g/L MnCl2 x 4 H2O, for 0.21 mM final.
ZnCl2 0.005 G_PER_L UTEX lists 0.005 g/L ZnCl2, for 0.037 mM final.
CoCl2 x 6 H2O 0.002 G_PER_L UTEX lists 0.002 g/L CoCl2 x 6 H2O, for 0.0084 mM final.
Na2MoO4 x 2 H2O 0.004 G_PER_L UTEX lists 0.004 g/L Na2MoO4 x 2 H2O, for 0.017 mM final.
UTEX adds 6 mL P-IV Metal Solution per liter.

Soilwater: GR+ Medium

Dose: 40 ML_PER_L

CultureMech:000225

UTEX lists 40 mL prepared Soilwater: GR+ Medium per liter.

Vitamin B12

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the Vitamin B12 stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock.
Vitamin B12 0.135 G_PER_L Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock.
UTEX adds 1 mL Vitamin B12 stock per liter.

Biotin Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the biotin stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX biotin stock.
Biotin 0.025 G_PER_L Converted from 0.005 g/200 mL in the UTEX biotin stock.
UTEX adds 1 mL Biotin Vitamin Solution per liter.

Thiamine Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the thiamine stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX thiamine stock.
Thiamine 0.335 G_PER_L Converted from 0.067 g/200 mL in the UTEX thiamine stock.
UTEX adds 1 mL Thiamine Vitamin Solution per liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: modified-bold-3n-medium, URL: https://utex.org/products/modified-bold-3n-medium
2026-03-13T06:18:53.446028Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000179
2026-03-17T19:45:04.378990+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Bolds 3N Medium" to "modified_bolds_3n_medium" for consistent matching
2026-05-16T09:02:56.043843+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.250663+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.872028+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 11 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_cr1_diatom_score35.py LINKED_UTEX_CR1_DIATOM_VARIANTS Added or refreshed reciprocal parent-child link for algae/Ag_Diatom_Medium.yaml.
2026-09-06T00:00:00-07:00 repair_utex_bristol_bold_score35.py RESOLVED_UTEX_BRISTOL_BOLD_SCORE35 UTEX prepares Modified Bold 3N Medium at pH 6.2 with the high-nitrate Bold 3N salts, P-IV Metal Solution, Soilwater: GR+ Medium, and Vitamin B12, Biotin, and Thiamine stocks.