modified_combo_medium

CultureMech:000181 algae COMPLEX LIQUID
Original name: Modified COMBO Medium

Overview

External ontology
UTEX:modified-combo-medium (source link unavailable) — Modified COMBO Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH
7.8
Notes
UTEX prepares Modified COMBO Medium at pH 7.8 with nitrate, calcium, magnesium, bicarbonate, silicate, phosphate, borate, chloride, Algal Trace Elements Solution, and B12, biotin, and thiamine stocks.

Composition

flowchart LR
    CultureMech_000181["modified_combo_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000181 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_NaNO3["NaNO3"]
    CultureMech_000181 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000181 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000181 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_NaHCO3["NaHCO3"]
    CultureMech_000181 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Na2SiO3_x_9_H2O["Na2SiO3 x 9 H2O"]
    CultureMech_000181 --> ing5_Na2SiO3_x_9_H2O
    CHEBI_132108["CHEBI:132108"]
    ing5_Na2SiO3_x_9_H2O -.-> CHEBI_132108
    ing6_K2HPO4["K2HPO4"]
    CultureMech_000181 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_H3BO3["H3BO3"]
    CultureMech_000181 --> ing7_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing7_H3BO3 -.-> CHEBI_33118
    ing8_KCl["KCl"]
    CultureMech_000181 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts the 1 L Modified COMBO recipe with approximately 900 mL dH2O and brings the final volume to 1 L.
2 NaNO3
NO3.Na 84.994 g/mol
Fisher BP360-500
10 MILLIMOLAR CHEBI:63005
sodium nitrate
 UTEX adds 10 mL/L of an 85.01 g/L NaNO3 stock, for 10 mM final.
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
Sigma C-3881
2.5 MILLIMOLAR CHEBI:86158
calcium chloride dihydrate
 UTEX adds 10 mL/L of a 36.76 g/L CaCl2 x 2 H2O stock, for 2.5 mM final.
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
Sigma 230391
1.5 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 UTEX adds 10 mL/L of a 36.97 g/L MgSO4 x 7 H2O stock, for 1.5 mM final.
5 NaHCO3
CHO3.Na 84.006 g/mol
Fisher S 233
1.5 MILLIMOLAR CHEBI:32139
sodium hydrogencarbonate
 UTEX adds 10 mL/L of a 12.6 g/L NaHCO3 stock, for 1.5 mM final.
6 Na2SiO3 x 9 H2O
9H2O.2Na.O3Si 284.198 g/mol
Sigma 307815
1 MILLIMOLAR CHEBI:132108
sodium silicate nonahydrate
 UTEX adds 10 mL/L of a 28.42 g/L Na2SiO3 x 9 H2O stock, for 1 mM final.
7 K2HPO4
HO4P.2K 174.174 g/mol
Sigma P 3786
0.5 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
 UTEX adds 1 mL/L of an 8.71 g/L K2HPO4 stock, for 0.5 mM final.
8 H3BO3
H3BO3 61.833 g/mol
Baker 0084
3.9 MILLIMOLAR CHEBI:33118
boric acid
 UTEX adds 10 mL/L of a 24 g/L H3BO3 stock, for 3.9 mM final.
9 KCl
Cl.K 74.551 g/mol
Fisher P 217
1 MILLIMOLAR CHEBI:32588
potassium chloride
 UTEX adds 10 mL/L of a 7.45 g/L KCl stock, for 1 mM final.

Preparation

  1. To approximately 900 mL dH2O, add all non-vitamin components in the listed order while stirring continuously.
  2. Adjust the medium to pH 7.8.
  3. Bring the total volume to 1 L with dH2O.
  4. Add the sterile vitamin stocks to the flask.
  5. Filter-sterilize the medium; UTEX notes not to autoclave it.
  6. Store at refrigerator temperature.

Solutions

Algal Trace Elements Solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings Algal Trace Elements Solution to 1 L with dH2O.
ZnSO4 x 7 H2O 0.023 G_PER_L Converted from 23 mg/L in the UTEX Algal Trace Elements Solution recipe.
MnSO4 x H2O 0.152 G_PER_L Converted from 152 mg/L in the UTEX Algal Trace Elements Solution recipe.
Na2MoO4 x 2 H2O 0.0073 G_PER_L Converted from 7.3 mg/L in the UTEX Algal Trace Elements Solution recipe.
CoSO4 x 7 H2O 0.014 G_PER_L Converted from 14 mg/L in the UTEX Algal Trace Elements Solution recipe.
CuCl2 x 2 H2O 0.0068 G_PER_L Converted from 6.8 mg/L in the UTEX Algal Trace Elements Solution recipe.
Fe(NH4)2(SO4)2 x 6 H2O 4.6 G_PER_L UTEX lists 4.6 g/L in the Algal Trace Elements Solution recipe.
Na2EDTA x 2 H2O 4.4 G_PER_L UTEX lists 4.4 g/L in the Algal Trace Elements Solution recipe.
UTEX adds 10 mL Algal Trace Elements Solution per liter of Modified COMBO.

Vitamin B12

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the Vitamin B12 stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock.
Vitamin B12 0.135 G_PER_L Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock.
UTEX adds 1 mL Vitamin B12 stock per liter of Modified COMBO.

Biotin Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the biotin stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX biotin stock.
Biotin 0.025 G_PER_L Converted from 0.005 g/200 mL in the UTEX biotin stock.
UTEX adds 1 mL Biotin Vitamin Solution per liter of Modified COMBO.

Thiamine Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the thiamine stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX thiamine stock.
Thiamine 0.335 G_PER_L Converted from 0.067 g/200 mL in the UTEX thiamine stock.
UTEX adds 1 mL Thiamine Vitamin Solution per liter of Modified COMBO.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: modified-combo-medium, URL: https://utex.org/products/modified-combo-medium
2026-03-13T06:18:53.457877Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000181
2026-03-17T19:45:04.389273+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified COMBO Medium" to "modified_combo_medium" for consistent matching
2026-05-16T09:02:56.056204+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.263518+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.887192+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 12 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_combo_diatom_score35.py RESOLVED_UTEX_COMBO_DIATOM_SCORE35 UTEX prepares Modified COMBO Medium at pH 7.8 with nitrate, calcium, magnesium, bicarbonate, silicate, phosphate, borate, chloride, Algal Trace Elements Solution, and B12, biotin, and thiamine stocks.
2026-09-06T00:00:00-07:00 repair_utex_combo_diatom_score35.py LINKED_UTEX_COMBO_DIATOM_VARIANTS Added or refreshed reciprocal parent-child link for algae/Combo_Diatom_Medium.yaml.