modified_desmidiacean_medium

CultureMech:000182 algae COMPLEX LIQUID
Original name: Modified Desmidiacean Medium

Overview

External ontology
UTEX:modified-desmidiacean-medium (source link unavailable) — Modified Desmidiacean Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares Modified Desmidiacean Medium from dH2O, nitrate, diammonium hydrogen phosphate, MgSO4, CaSO4 saturated solution, prepared Soilwater: GR+ Medium, prepared Soilwater: Peat Medium, MWC Metal Solution, and one sterile barley grain per test tube.

Composition

flowchart LR
    CultureMech_000182["modified_desmidiacean_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000182 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_KNO3["KNO3"]
    CultureMech_000182 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2__NH4_2HPO4["(NH4)2HPO4"]
    CultureMech_000182 --> ing2__NH4_2HPO4
    CHEBI_63051["CHEBI:63051"]
    ing2__NH4_2HPO4 -.-> CHEBI_63051
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000182 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Barley_grains_autoclaved["Barley grains autoclaved"]
    CultureMech_000182 --> ing4_Barley_grains_autoclaved

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts with approximately 900 mL dH2O and brings Modified Desmidiacean Medium to 1 L with dH2O.
2 KNO3
K.NO3 101.102 g/mol
Baker 3190
0.9 MILLIMOLAR CHEBI:63043
potassium nitrate
 UTEX adds 10 mL/L of a 1 g/100 mL KNO3 stock, for 0.9 mM final.
3 (NH4)2HPO4
2H4N.HO4P 132.056 g/mol
Fisher A686
0.075 MILLIMOLAR CHEBI:63051
diammonium hydrogen phosphate
 UTEX adds 5 mL/L of a 0.2 g/100 mL (NH4)2HPO4 stock, for 0.075 mM final.
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
Sigma 230391
3.3 MICROMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 UTEX adds 10 mL/L of a 0.1 g/100 mL stock, for 3.3 uM final.
5 Barley grains autoclaved 1 grain per test tube VARIABLE UTEX adds one sterile barley grain per test tube after cooling.

Preparation

  1. To approximately 900 mL dH2O, add the first seven components in the order specified while stirring continuously.
  2. Bring the total volume to 1 L with dH2O.
  3. Cover and autoclave the medium.
  4. Allow the medium to cool, then add one sterile barley grain per tube.
  5. Store at refrigerator temperature.

Solutions

CaSO4 x 2 H2O saturated solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
CaSO4 x 2 H2O saturated VARIABLE UTEX lists saturated CaSO4 x 2 H2O solution, Mallinckrodt 4300.
UTEX adds 10 mL CaSO4 x 2 H2O saturated solution per liter.

Soilwater: GR+ Medium

Dose: 20 ML_PER_L

CultureMech:000225

UTEX lists 20 mL prepared Soilwater: GR+ Medium per liter.

Soilwater: Peat Medium

Dose: 10 ML_PER_L

CultureMech:000230

UTEX lists 10 mL prepared Soilwater: Peat Medium per liter.

MWC Metal Solution

Dose: 5 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings MWC Metal Solution to 1 L with dH2O.
Na2EDTA x 2 H2O 0.44 G_PER_L UTEX lists 0.44 g/L Na2EDTA x 2 H2O.
H3BO3 0.097 G_PER_L UTEX lists 0.097 g/L H3BO3.
FeCl3 x 6 H2O 0.315 G_PER_L UTEX lists 0.315 g/L FeCl3 x 6 H2O.
MnCl2 x 4 H2O 0.018 G_PER_L UTEX lists 0.018 g/L MnCl2 x 4 H2O.
ZnSO4 x 7 H2O 0.002 G_PER_L UTEX lists 0.002 g/L ZnSO4 x 7 H2O.
CoCl2 x 6 H2O 0.001 G_PER_L UTEX lists 0.001 g/L CoCl2 x 6 H2O.
Na2MoO4 x 2 H2O 0.0006 G_PER_L UTEX lists 0.0006 g/L Na2MoO4 x 2 H2O.
UTEX adds 5 mL MWC Metal Solution per liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: modified-desmidiacean-medium, URL: https://utex.org/products/modified-desmidiacean-medium
2026-03-13T06:18:53.464152Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000182
2026-03-17T19:45:04.394930+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Desmidiacean Medium" to "modified_desmidiacean_medium" for consistent matching
2026-05-16T09:02:56.062786+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.270617+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.895449+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 8 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_modified_ldm_score35.py RESOLVED_UTEX_MODIFIED_LDM_SCORE35 UTEX prepares Modified Desmidiacean Medium from dH2O, nitrate, diammonium hydrogen phosphate, MgSO4, CaSO4 saturated solution, prepared Soilwater: GR+ Medium, prepared Soilwater: Peat Medium, MWC Metal Solution, and one sterile barley grain per test tube.