ochromonas_medium

CultureMech:000184 algae COMPLEX LIQUID
Original name: Ochromonas Medium

Overview

External ontology
UTEX:ochromonas-medium (source link unavailable) — Ochromonas Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares Ochromonas Medium from dH2O, glucose, tryptone, yeast extract, and Liver extract infusion.

Composition

flowchart LR
    CultureMech_000184["ochromonas_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000184 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_Glucose["Glucose"]
    CultureMech_000184 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234
    ing2_Tryptone["Tryptone"]
    CultureMech_000184 --> ing2_Tryptone
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_000184 --> ing3_Yeast_extract

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
CHEBI:15377
water
 Start with approximately 900 mL dH2O, then bring the supplemented medium to 1 L total with additional dH2O. The exact combined water dose is not specified.
2 Glucose
C6H12O6 180.156 g/mol
5.6 MILLIMOLAR CHEBI:17234
glucose
 UTEX lists 1 g/L Glucose, with BACTO-dextrose or Sigma D 9634 as the source for 5.6 mM final.
3 Tryptone
Sigma T 9410
1 G_PER_L MICRO:0000182 (source link unavailable)
Tryptone
 UTEX lists 1 g/L Tryptone, Sigma T 9410.
4 Yeast extract 1 G_PER_L FOODON:03315426
Yeast extract
 UTEX lists 1 g/L Yeast extract, Bacto/Difco.

Preparation

  1. To approximately 900 mL dH2O, add each component in the order specified while stirring continuously.
  2. Bring the total volume to 1 L with dH2O.
  3. Autoclave the medium.
  4. Store at refrigerator temperature.

Solutions

Liver extract infusion

Dose: 40 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O Start with approximately 40 mL dH2O and bring the liver-extract preparation to 50 mL total with additional dH2O before heating and filtration. The exact combined water dose is not specified.
Liver extract 50 G_PER_L (per liter of prepared stock before heating and filtration) Use 2.5 g liver extract in 50 mL total stock before heating and filtration, equivalent to a 50 g/L preparation input. The recovered filtrate concentration is not measured by this recipe.
UTEX adds 40 mL Liver extract infusion per liter of Ochromonas Medium. Prepare the stock to 50 mL, heat in a 50 C water bath for approximately 1 hour, then in an 80 C water bath for 3-5 minutes, and filter. Its 50 g/L component amount is a pre-filtration preparation input.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: ochromonas-medium, URL: https://utex.org/products/ochromonas-medium
2026-03-13T06:18:53.476157Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000184
2026-03-17T19:45:04.405515+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Ochromonas Medium" to "ochromonas_medium" for consistent matching
2026-05-16T09:02:56.074408+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.284017+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.911664+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 4 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_organic_score35.py RESOLVED_UTEX_ORGANIC_SCORE35 UTEX prepares Ochromonas Medium from dH2O, glucose, tryptone, yeast extract, and Liver extract infusion.
2026-10-10T21:08:21.569593+00:00 codex CURATED_UTEX_ORGANIC_MEDIUM_INPUT_BASES_AND_CONCENTRATION_EVIDENCE Exact source-URL and snippet evidence for input doses, optional agar and retained concentrations. Chemical identity mappings and unrelated growth/role claims are unchanged, not independently certified. No taxa-media association added.