p49_medium

CultureMech:000185 algae COMPLEX LIQUID
Original name: P49 Medium

Overview

External ontology
UTEX:p49-medium (source link unavailable) — P49 Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH
7.0
Notes
UTEX prepares P49 Medium at pH 7.0 from dH2O, magnesium sulfate, sodium glycerophosphate, potassium chloride, glycylglycine, P-IV Metal Solution, ammonium nitrate, calcium chloride, yeast extract, Tryptone, and three sterile vitamin stocks.

Composition

flowchart LR
    CultureMech_000185["p49_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000185 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000185 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_Na2glycerophosphate_x_5_H2O["Na2glycerophosphate x 5 H2O"]
    CultureMech_000185 --> ing2_Na2glycerophosphate_x_5_H2O
    CHEBI_15978["CHEBI:15978"]
    ing2_Na2glycerophosphate_x_5_H2O -.-> CHEBI_15978
    ing3_KCl["KCl"]
    CultureMech_000185 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Glycylglycine["Glycylglycine"]
    CultureMech_000185 --> ing4_Glycylglycine
    CHEBI_17201["CHEBI:17201"]
    ing4_Glycylglycine -.-> CHEBI_17201
    ing5_NH4NO3["NH4NO3"]
    CultureMech_000185 --> ing5_NH4NO3
    CHEBI_63038["CHEBI:63038"]
    ing5_NH4NO3 -.-> CHEBI_63038
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000185 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_000185 --> ing7_Yeast_extract
    ing8_Tryptone["Tryptone"]
    CultureMech_000185 --> ing8_Tryptone

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts the 1 L P49 Medium recipe with approximately 900 mL dH2O.
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.16 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 UTEX adds 1 mL/L of a 4 g/100 mL dH2O stock, for 0.16 mM final.
3 Na2glycerophosphate x 5 H2O
C3H9O6P 172.073 g/mol
0.16 MILLIMOLAR CHEBI:15978
sn-glycerol 3-phosphate
 UTEX adds 1 mL/L of a 5 g/100 mL dH2O stock, for 0.16 mM final.
4 KCl
Cl.K 74.551 g/mol
0.67 MILLIMOLAR CHEBI:32588
potassium chloride
 UTEX adds 1 mL/L of a 5 g/100 mL dH2O stock, for 0.67 mM final.
5 Glycylglycine
C4H8N2O3 132.119 g/mol
3.8 MILLIMOLAR CHEBI:17201
glycylglycine
 UTEX adds 10 mL/L of a 5 g/100 mL dH2O stock, for 3.8 mM final.
6 NH4NO3
H4N.NO3 80.043 g/mol
1.2 MILLIMOLAR CHEBI:63038
ammonium nitrate
 UTEX adds 10 mL/L of a 1 g/100 mL dH2O stock, for 1.2 mM final.
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.5 MILLIMOLAR CHEBI:86158
calcium chloride dihydrate
 UTEX adds 10 mL/L of a 0.74 g/100 mL dH2O stock, for 0.5 mM final.
8 Yeast extract 0.2 G_PER_L FOODON:03315426
yeast extract
 UTEX lists 0.2 g/L yeast extract.
9 Tryptone 0.4 G_PER_L MICRO:0000182 (source link unavailable)
Tryptone
 UTEX lists 0.4 g/L Tryptone.

Preparation

  1. To approximately 900 mL dH2O, add each component except vitamins in the order specified while stirring continuously.
  2. Adjust the medium to pH 7.0.
  3. Bring the total volume to 1 L with dH2O.
  4. Cover and autoclave the medium.
  5. Allow the medium to cool, then add sterile vitamins.
  6. Store at refrigerator temperature.

Solutions

P-IV Metal Solution

Dose: 6 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings P-IV Metal Solution to 1 L with dH2O.
Na2EDTA x 2 H2O 0.75 G_PER_L UTEX lists 0.75 g/L Na2EDTA x 2 H2O, 2 mM final, and dissolves it fully before the other P-IV components.
FeCl3 x 6 H2O 0.097 G_PER_L UTEX lists 0.097 g/L FeCl3 x 6 H2O, for 0.36 mM final.
MnCl2 x 4 H2O 0.041 G_PER_L UTEX lists 0.041 g/L MnCl2 x 4 H2O, for 0.21 mM final.
ZnCl2 0.005 G_PER_L UTEX lists 0.005 g/L ZnCl2, for 0.037 mM final.
CoCl2 x 6 H2O 0.002 G_PER_L UTEX lists 0.002 g/L CoCl2 x 6 H2O, for 0.0084 mM final.
Na2MoO4 x 2 H2O 0.004 G_PER_L UTEX lists 0.004 g/L Na2MoO4 x 2 H2O, for 0.017 mM final.
UTEX adds 6 mL P-IV Metal Solution per liter.

Vitamin B12

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the Vitamin B12 stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock.
Vitamin B12 0.135 G_PER_L Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock.
UTEX adds 1 mL Vitamin B12 stock per liter.

Biotin Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the biotin stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX biotin stock.
Biotin 0.025 G_PER_L Converted from 0.005 g/200 mL in the UTEX biotin stock.
UTEX adds 1 mL Biotin Vitamin Solution per liter.

Thiamine Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the thiamine stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX thiamine stock.
Thiamine 0.335 G_PER_L Converted from 0.067 g/200 mL in the UTEX thiamine stock.
UTEX adds 1 mL Thiamine Vitamin Solution per liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: p49-medium, URL: https://utex.org/products/p49-medium
2026-03-13T06:18:53.482458Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000185
2026-03-17T19:45:04.410903+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "P49 Medium" to "p49_medium" for consistent matching
2026-05-16T09:02:56.080749+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.290682+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.919489+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 12 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_volvox_p49_score35.py RESOLVED_UTEX_VOLVOX_P49_SCORE35 UTEX prepares P49 Medium at pH 7.0 from dH2O, magnesium sulfate, sodium glycerophosphate, potassium chloride, glycylglycine, P-IV Metal Solution, ammonium nitrate, calcium chloride, yeast extract, Tryptone, and three sterile vitamin stocks.