polytomella_medium (CultureMech:000186)

Overview

External ontology

UTEX:polytomella-medium (source link unavailable) — Polytomella Medium

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

UTEX prepares Polytomella Medium from dH2O, yeast extract, tryptone, and sodium acetate.

Composition

flowchart LR
    CultureMech_000186["polytomella_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000186 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000186 --> ing1_Yeast_extract
    ing2_Tryptone["Tryptone"]
    CultureMech_000186 --> ing2_Tryptone
    ing3_Sodium_acetate["Sodium acetate"]
    CultureMech_000186 --> ing3_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Sodium_acetate -.-> CHEBI_32954

Ingredients (4)

#	Ingredient	Concentration	Ontology	Notes
1	dH2O H2O 18.015 g/mol		CHEBI:15377 water	Start with approximately 950 mL dH2O, then bring the supplemented medium to 1 L total with additional dH2O. The exact combined water dose is not specified.
2	Yeast extract	1 G_PER_L	FOODON:03315426 Yeast extract	UTEX lists 1 g/L Yeast extract, Bacto/Difco.
3	Tryptone Sigma T 9410	1 G_PER_L	MICRO:0000182 (source link unavailable) Tryptone	UTEX lists 1 g/L Tryptone, Sigma T 9410.
4	Sodium acetate C2H3O2.Na 82.034 g/mol Fisher BP 333	24.4 MILLIMOLAR	CHEBI:32954 sodium acetate	UTEX lists 2 g/L Sodium acetate, for 24.4 mM final.

Preparation

To approximately 950 mL dH2O, add each component in the order specified while stirring continuously.
Bring the total volume to 1 L with dH2O.
Cover and autoclave the medium.
Store at refrigerator temperature.

Target organisms

No target organisms are recorded.

Recipe variants

Name
Polytomella Medium with agar
Relationship
SUPPLEMENTED_VARIANT
Description
Optional source-listed agar formulation; the parent remains liquid.
Modifications
- After making up the liquid recipe to 1 L, add 15 g agar; do not mix.
Purpose
Preserve the separately specified solid formulation
Evidence
- Reference
  https://utex.org/products/polytomella-medium
  Supports
  SUPPORT
  Snippet
  For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  Explanation
  Supports variants[0].modifications only. The source labels this the 1.5% agar formulation; no new measured final volume after agar addition is claimed. No agar is added to the liquid parent.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	utex-import	Imported from UTEX Culture Collection	Source ID: polytomella-medium, URL: https://utex.org/products/polytomella-medium
2026-03-13T06:18:53.487970Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000186
2026-03-17T19:45:04.415841+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Polytomella Medium" to "polytomella_medium" for consistent matching
2026-05-16T09:02:56.086257+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.296518+00:00	migrate_preparation_steps.py	MIGRATED_PREPARATION_STEPS	instruction->action+description for 9 step(s)
2026-08-30T00:58:30.926608+00:00	repair_utex_ingredients.py	REPAIRED_UTEX_COMPOSITION	Restored 3 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00	repair_utex_organic_score35.py	RESOLVED_UTEX_ORGANIC_SCORE35	UTEX prepares Polytomella Medium from dH2O, yeast extract, tryptone, and sodium acetate.
2026-10-10T21:08:21.569593+00:00	codex	CURATED_UTEX_ORGANIC_MEDIUM_INPUT_BASES_AND_CONCENTRATION_EVIDENCE	Exact source-URL and snippet evidence for input doses, optional agar and retained concentrations. Chemical identity mappings and unrelated growth/role claims are unchanged, not independently certified. No taxa-media association added.