porphryridium_medium

CultureMech:000187 algae COMPLEX LIQUID
Original name: Porphryridium Medium

Overview

External ontology
UTEX:porphyridium-medium (source link unavailable) — Porphyridium Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares Porphyridium Medium from dH2O, non-sterilized seawater, prepared Soilwater: GR+ Medium, yeast extract, and tryptone.

Composition

flowchart LR
    CultureMech_000187["porphryridium_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000187 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_Seawater["Seawater"]
    CultureMech_000187 --> ing1_Seawater
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_000187 --> ing2_Yeast_extract
    ing3_Tryptone["Tryptone"]
    CultureMech_000187 --> ing3_Tryptone

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts the 1 L Porphyridium Medium recipe with approximately 350 mL dH2O.
2 Seawater 500 ML_PER_L ENVO:00002149
sea water
 UTEX lists 500 mL/L non-sterilized Seawater.
3 Yeast extract 1 G_PER_L FOODON:03315426
Yeast extract
 UTEX lists 1 g/L Yeast extract, Bacto/Difco.
4 Tryptone
Sigma T 9410
1 G_PER_L MICRO:0000182 (source link unavailable)
Tryptone
 UTEX lists 1 g/L Tryptone, Sigma T 9410.

Preparation

  1. To approximately 350 mL dH2O, add each component in the order specified while stirring continuously.
  2. Bring the total volume to 1 L with dH2O.
  3. Cover and autoclave the medium.
  4. Store at refrigerator temperature.

Solutions

Soilwater: GR+ Medium

Dose: 100 ML_PER_L

CultureMech:000225

UTEX lists 100 mL prepared Soilwater: GR+ Medium per liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: porphyridium-medium, URL: https://utex.org/products/porphyridium-medium
2026-03-13T06:18:53.492869Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000187
2026-03-17T19:45:04.420472+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Porphryridium Medium" to "porphryridium_medium" for consistent matching
2026-05-16T09:02:56.090921+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.301795+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.933748+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 4 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_organic_score35.py RESOLVED_UTEX_ORGANIC_SCORE35 UTEX prepares Porphyridium Medium from dH2O, non-sterilized seawater, prepared Soilwater: GR+ Medium, yeast extract, and tryptone.