artificial_seawater

Overview

Applications

• Algae cultivation
• Phytoplankton culture
• Microalgae research

pH range

{'min': 8.0, 'max': 8.0}

Notes

Full recipe available at http://sagdb.uni-goettingen.de/culture_media/25 Artificial Seawater Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/25%20Artificial%20Seawater%20Medium.pdf on 2026-08-25 (SHA-256 0735fc07df10bd546b0e10fe21d3ee8cfad830775d7ae8e0afbba9137eb0a128).

Composition

flowchart LR
    CultureMech_000189["artificial_seawater"]
    ing0_NaCl["NaCl"]
    CultureMech_000189 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_TRIS["TRIS"]
    CultureMech_000189 --> ing1_TRIS
    ing2_Deionized_or_distilled_water["Deionized or distilled water"]
    CultureMech_000189 --> ing2_Deionized_or_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Deionized_or_distilled_water -.-> CHEBI_15377

Ingredients (3)

Preparation

1. Dissolve NaCl and TRIS in water, then add the mineral and trace stocks.
2. Adjust to pH 8.0 with 1 N HCl.
3. Autoclave the base medium.
4. Cool and add sterile vitamin B12 and vitamin B1 stocks.
5. For half-strength ASM15 use 15 g/L NaCl; agar at 15 g/L is an optional solid variant.

Solutions

Target organisms

No target organisms are recorded.

Provenance