bold_modified_basal (CultureMech:000194)

Overview

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

pH range

{'min': 6.4, 'max': 6.4}

Notes

Full recipe available at http://sagdb.uni-goettingen.de/culture_media/26 Bold Modified Basal Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/26%20Bold%20Modified%20Basal%20Medium.pdf on 2026-08-25 (SHA-256 71ff69ab6b5c184ad5777e2c99ebf9be353c536cdba664871edd3bb747378a7f).

Composition

flowchart LR
    CultureMech_000194["bold_modified_basal"]
    ing0_Deionized_or_distilled_water["Deionized or distilled water"]
    CultureMech_000194 --> ing0_Deionized_or_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Deionized_or_distilled_water -.-> CHEBI_15377

Ingredients (1)

#	Ingredient	Concentration	Ontology	Notes
1	Deionized or distilled water H2O 18.015 g/mol	940 ML_PER_L	CHEBI:15377 water

Preparation

Add six 10 mL mineral-stock portions to 940 mL water.
Autoclave the mineral base.
Add filter-sterilized vitamin and PIV metal solutions after cooling.
The 3NBBM+V variant uses 30 rather than 10 mL/L NaNO3 stock.

Solutions

NaNO3 stock solution

Dose: 10 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
NaNO3	25 G_PER_L

Source stock is 10 g in 400 mL.

CaCl2 x 2 H2O stock solution

Dose: 10 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
CaCl2 x 2 H2O	2.5 G_PER_L

Source stock is 1 g in 400 mL.

MgSO4 x 7 H2O stock solution

Dose: 10 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
MgSO4 x 7 H2O	7.5 G_PER_L

Source stock is 3 g in 400 mL.

K2HPO4 x 3 H2O stock solution

Dose: 10 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
K2HPO4 x 3 H2O	7.5 G_PER_L

Source stock is 3 g in 400 mL.

KH2PO4 stock solution

Dose: 10 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
KH2PO4	17.5 G_PER_L

Source stock is 7 g in 400 mL.

NaCl stock solution

Dose: 10 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
NaCl	2.5 G_PER_L

Source stock is 1 g in 400 mL.

Thiamine stock solution

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Thiamine	1 G_PER_L

Biotin stock solution

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Biotin	0.25 MG_PER_L

Vitamin B12 stock solution

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Vitamin B12	0.15 MG_PER_L

PIV metal solution

Dose: 6 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Na2EDTA	750 MG_PER_L	Disodium EDTA; hydration state unspecified.
FeCl3 x 6 H2O	97 MG_PER_L
MnCl2 x 4 H2O	41 MG_PER_L
ZnCl2	5 MG_PER_L
CoCl2 x 6 H2O	2 MG_PER_L
Na2MoO4 x 2 H2O	4 MG_PER_L
Deionized or distilled water	1 L

Dissolve EDTA fully, then add salts in the source order.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	sag-import	Imported from SAG Culture Collection	Source ID: Bold_Modified_Basal, PDF URL: http://sagdb.uni-goettingen.de/culture_media/26 Bold Modified Basal Medium.pdf
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added placeholder ingredients for missing composition
2026-02-03T11:26:43.764273	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.513977Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000194
2026-03-17T19:45:04.440780+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Bold_Modified_Basal" to "bold_modified_basal" for consistent matching
2026-05-16T09:02:56.389484+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.015897+00:00	curate_placeholder_composition.py	REMOVED_PLACEHOLDER_INGREDIENT	Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00	repair_sag_missing_compositions.py	RESTORED_SAG_SOURCE_COMPOSITION	Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM.
2026-09-07T00:00:00-07:00	repair_sag_missing_compositions.py	TAGGED_SAG_RESTORED_COMPOSITION_CURATED	Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags.
2026-09-13T00:00:00-07:00	repair_sag_sources_score10.py	NORMALIZED_SAG_SOURCE_SCORE10	Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring.