bold_modified_basal_tom

CultureMech:000195 algae COMPLEX LIQUID
Original name: Bold_Modified_Basal&TOM

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH range
{'min': 7.1, 'max': 7.1}
Notes
Full recipe available at http://sagdb.uni-goettingen.de/culture_media/30 Bold Modified Basal Medium&TOM.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/30%20Bold%20Modified%20Basal%20Medium%26TOM.pdf on 2026-08-25 (SHA-256 e6d2940c4685e5c2c16fbb1526d3f7f0f7160747acd37746b50491851e6577c5).

Composition

flowchart LR
    CultureMech_000195["bold_modified_basal_tom"]
    ing0_HEPES["HEPES"]
    CultureMech_000195 --> ing0_HEPES
    CHEBI_42334["CHEBI:42334"]
    ing0_HEPES -.-> CHEBI_42334
    ing1_Glucose["Glucose"]
    CultureMech_000195 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234
    ing2_Proteose_Peptone["Proteose Peptone"]
    CultureMech_000195 --> ing2_Proteose_Peptone
    ing3_Deionized_or_distilled_water["Deionized or distilled water"]
    CultureMech_000195 --> ing3_Deionized_or_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Deionized_or_distilled_water -.-> CHEBI_15377

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 HEPES
C8H18N2O4S 238.309 g/mol
0.715 G_PER_L CHEBI:42334
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
2 Glucose
C6H12O6 180.156 g/mol
15 G_PER_L CHEBI:17234
glucose
3 Proteose Peptone 20 G_PER_L MICRO:0000180 (source link unavailable)
proteose peptone
4 Deionized or distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
 Make the MBB base to 1 L before TOM supplementation.

Preparation

  1. Add 10 mL of each mineral stock and 1 mL of each trace stock; make to 1 L.
  2. Add 0.715 g HEPES, 1.5% glucose, and 2% proteose peptone for TOM.
  3. Adjust TOM to pH 7.1.
  4. The PDF lists a vitamin stock but gives no unambiguous final addition rate, so it is not asserted.
  5. Autoclave following the SAG general preparation guidance.

Solutions

NaCl stock solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
NaCl 2.5 G_PER_L
Source stock is 1.25 g in 500 mL.

CaCl2 x 2 H2O stock solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
CaCl2 x 2 H2O 2.5 G_PER_L
Source stock is 1.25 g in 500 mL.

KNO3 stock solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
KNO3 50 G_PER_L
Source stock is 25 g in 500 mL.

MgSO4 x 7 H2O stock solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
MgSO4 x 7 H2O 6.5 G_PER_L
Source stock is 3.25 g in 500 mL.

(NH4)2HPO4 stock solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
(NH4)2HPO4 25 G_PER_L
Source stock is 12.5 g in 500 mL.

MBB trace element stock I

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
KOH 30 G_PER_L
EDTA 50 G_PER_L
Deionized or distilled water 1 L

MBB trace element stock II

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 4.98 G_PER_L
Deionized or distilled water 1 L
The source specifies dissolving with sulfuric acid but gives no amount.

MBB trace element stock III

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
H3BO3 11.04 G_PER_L
Deionized or distilled water 1 L

MBB trace element stock IV

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
ZnSO4 x 7 H2O 17.64 G_PER_L
MnCl2 x 4 H2O 1.44 G_PER_L
NaMoO4 0.72 G_PER_L Source formula retained; no exact SSSOM mapping was asserted.
CuSO4 x 5 H2O 1.58 G_PER_L
Co(NO3)2 x 6 H2O 0.50 G_PER_L
Deionized or distilled water 1 L
The source specifies dissolving with sulfuric acid but gives no amount.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 sag-import Imported from SAG Culture Collection Source ID: Bold_Modified_Basal&TOM, PDF URL: http://sagdb.uni-goettingen.de/culture_media/30 Bold Modified Basal Medium&TOM.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.766540 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.516901Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000195
2026-03-17T19:45:04.443398+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Bold_Modified_Basal&TOM" to "bold_modified_basal_tom" for consistent matching
2026-05-16T09:02:56.392599+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.017518+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_sag_missing_compositions.py RESTORED_SAG_SOURCE_COMPOSITION Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM.
2026-09-07T00:00:00-07:00 repair_sag_missing_compositions.py TAGGED_SAG_RESTORED_COMPOSITION_CURATED Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags.
2026-09-13T00:00:00-07:00 repair_sag_sources_score10.py NORMALIZED_SAG_SOURCE_SCORE10 Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring.