Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at http://sagdb.uni-goettingen.de/culture_media/06 Brackish water medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/06%20Brackish%20water%20medium.pdf on 2026-08-25 (SHA-256 5a92afd0df065d50f348c964857f98e1be43217ecc6173911a7762aa42fe85ae).
Composition
flowchart LR
CultureMech_000196["brackish_water_medium"]
ing0_Deionized_or_distilled_water["Deionized or distilled water"]
CultureMech_000196 --> ing0_Deionized_or_distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Deionized_or_distilled_water -.-> CHEBI_15377
ing1_Filtered_seawater["Filtered seawater"]
CultureMech_000196 --> ing1_Filtered_seawater
ing2_Vitamin_B12["Vitamin B12"]
CultureMech_000196 --> ing2_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing2_Vitamin_B12 -.-> CHEBI_176843
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Deionized or distilled water
H2O 18.015 g/mol
|
450 ML_PER_L |
CHEBI:15377
water
|
|
| 2 | Filtered seawater | 455 ML_PER_L |
MICRO:0001773 (source link unavailable)
filtered seawater
|
|
| 3 | Vitamin B12 | 0.005 MG_PER_L |
CHEBI:176843
vitamin B12
|
Add in sterile solution after cooling. |
Preparation
- Combine the stocks with equal parts water and filtered seawater.
- Autoclave soil extract separately; steam or autoclave the remaining medium.
- Unite after cooling and add sterile vitamin B12.
- Silicate and selenite variants are described separately in the source and are not asserted here.
Solutions
KNO3 stock solution
Dose: 20 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| KNO3 | 10 G_PER_L |
K2HPO4 stock solution
Dose: 20 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| K2HPO4 | 1 G_PER_L |
MgSO4 x 7 H2O stock solution
Dose: 20 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgSO4 x 7 H2O | 1 G_PER_L |
Soil extract
Dose: 30 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Garden or leaf soil | 1 VARIABLE | Fill one third of a vessel; the source gives a geometric amount. |
| Deionized or distilled water | 1 VARIABLE | Add until water stands 5 cm above the soil. |
Steam for 1 hour twice at a 24-hour interval, decant and centrifuge, then autoclave aliquots for 20 minutes at 121 C. Concentrations are unspecified by the source.
SAG medium 1 micronutrient solution
Dose: 5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| ZnSO4 x 7 H2O | 1 MG_PER_L | |
| MnSO4 x 4 H2O | 2 MG_PER_L | |
| H3BO3 | 10 MG_PER_L | |
| Co(NO3)2 x 6 H2O | 1 MG_PER_L | |
| Na2MoO4 x 2 H2O | 1 MG_PER_L | |
| CuSO4 x 5 H2O | 0.005 MG_PER_L | |
| FeSO4 x 7 H2O | 700 MG_PER_L | |
| EDTA | 800 MG_PER_L | |
| Deionized or distilled water | 981 ML_PER_L |
Stock concentrations are calculated from the source primary-stock volumes into the 1 L applied solution. Prepare as two separately autoclaved solutions and unite after cooling.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | sag-import | Imported from SAG Culture Collection | Source ID: Brackish_water_medium, PDF URL: http://sagdb.uni-goettingen.de/culture_media/06 Brackish water medium.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.768510 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.519934Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000196 |
| 2026-03-17T19:45:04.446008+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Brackish_water_medium" to "brackish_water_medium" for consistent matching |
| 2026-05-16T09:02:56.395673+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-06T07:56:36.019207+00:00 | curate_placeholder_composition.py | REMOVED_PLACEHOLDER_INGREDIENT | Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175). |
| 2026-08-25T00:00:00-07:00 | repair_sag_missing_compositions.py | RESTORED_SAG_SOURCE_COMPOSITION | Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM. |
| 2026-09-07T00:00:00-07:00 | repair_sag_missing_compositions.py | TAGGED_SAG_RESTORED_COMPOSITION_CURATED | Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags. |
| 2026-09-13T00:00:00-07:00 | repair_sag_sources_score10.py | NORMALIZED_SAG_SOURCE_SCORE10 | Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring. |