cyanidium

CultureMech:000198 algae COMPLEX LIQUID
Original name: Cyanidium

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH range
{'min': 4.0, 'max': 4.0}
Notes
Full recipe available at http://sagdb.uni-goettingen.de/culture_media/17 Cyanidium Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/17%20Cyanidium%20Medium.pdf on 2026-08-25 (SHA-256 d304e35db6b75fc8b639518cf9dc299954b872718d15f1caeb02ca062039976f).

Composition

flowchart LR
    CultureMech_000198["cyanidium"]
    ing0_Deionized_or_distilled_water["Deionized or distilled water"]
    CultureMech_000198 --> ing0_Deionized_or_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Deionized_or_distilled_water -.-> CHEBI_15377

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 Deionized or distilled water
H2O 18.015 g/mol
830 ML_PER_L CHEBI:15377
water

Preparation

  1. Prepare the stock solutions and combine them at the stated amounts.
  2. Autoclave at 121 C for 15 minutes following the SAG general preparation guidance.
  3. Vitamin B1, vitamin B12, and combined B1/B12 variants are described separately and are not asserted here.

Solutions

(NH4)2SO4 stock solution

Dose: 100 ML_PER_L

Stock composition
IngredientConcentrationNotes
(NH4)2SO4 10 G_PER_L

K2HPO4 stock solution

Dose: 20 ML_PER_L

Stock composition
IngredientConcentrationNotes
K2HPO4 1 G_PER_L

MgSO4 x 7 H2O stock solution

Dose: 20 ML_PER_L

Stock composition
IngredientConcentrationNotes
MgSO4 x 7 H2O 1 G_PER_L

Soil extract

Dose: 30 ML_PER_L

Stock composition
IngredientConcentrationNotes
Garden or leaf soil 1 VARIABLE Fill one third of a vessel; the source gives a geometric amount.
Deionized or distilled water 1 VARIABLE Add until water stands 5 cm above the soil.
Steam for 1 hour twice at a 24-hour interval, decant and centrifuge, then autoclave aliquots for 20 minutes at 121 C. Concentrations are unspecified by the source.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 sag-import Imported from SAG Culture Collection Source ID: Cyanidium, PDF URL: http://sagdb.uni-goettingen.de/culture_media/17 Cyanidium Medium.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.772334 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.525580Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000198
2026-03-17T19:45:04.452035+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Cyanidium" to "cyanidium" for consistent matching
2026-05-16T09:02:56.445735+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.023821+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_sag_missing_compositions.py RESTORED_SAG_SOURCE_COMPOSITION Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM.
2026-09-07T00:00:00-07:00 repair_sag_missing_compositions.py TAGGED_SAG_RESTORED_COMPOSITION_CURATED Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags.
2026-09-13T00:00:00-07:00 repair_sag_sources_score10.py NORMALIZED_SAG_SOURCE_SCORE10 Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring.