desmidiacean (CultureMech:000199)

Overview

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

Full recipe available at http://sagdb.uni-goettingen.de/culture_media/07 Desmidiacean Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/07%20Desmidiacean%20Medium.pdf on 2026-08-25 (SHA-256 6d7b4d0f2c2e68d631ccdb0a40666a7981407d46f80de6864c3b9ea28c56b3b5).

Composition

flowchart LR
    CultureMech_000199["desmidiacean"]
    ing0_Deionized_or_distilled_water["Deionized or distilled water"]
    CultureMech_000199 --> ing0_Deionized_or_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Deionized_or_distilled_water -.-> CHEBI_15377
    ing1_Vitamin_B12["Vitamin B12"]
    CultureMech_000199 --> ing1_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing1_Vitamin_B12 -.-> CHEBI_176843

Ingredients (2)

#	Ingredient	Concentration	Ontology	Notes
1	Deionized or distilled water H2O 18.015 g/mol	930 ML_PER_L	CHEBI:15377 water
2	Vitamin B12	0.005 MG_PER_L	CHEBI:176843 vitamin B12	Add in sterile solution after cooling.

Preparation

Prepare the stock solutions and combine them at the stated amounts.
Autoclave at 121 C for 15 minutes following the SAG general preparation guidance.
Cool before adding sterile heat-labile components.
Add sterile vitamin B12; a separate source variant also adds vitamin B1.

Solutions

KNO3 stock solution

Dose: 10 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
KNO3	10 G_PER_L

(NH4)2HPO4 stock solution

Dose: 5 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
(NH4)2HPO4	2 G_PER_L

MgSO4 x 7 H2O stock solution

Dose: 10 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
MgSO4 x 7 H2O	1 G_PER_L

Saturated CaSO4 solution

Dose: 10 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
CaSO4	1 VARIABLE	Prepare a saturated solution; the source gives no mass concentration.

Soil extract

Dose: 20 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Garden or leaf soil	1 VARIABLE	Fill one third of a vessel; the source gives a geometric amount.
Deionized or distilled water	1 VARIABLE	Add until water stands 5 cm above the soil.

Steam for 1 hour twice at a 24-hour interval, decant and centrifuge, then autoclave aliquots for 20 minutes at 121 C. Concentrations are unspecified by the source.

Peat extract

Dose: 10 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Peat soil	1 VARIABLE	Fill one third of a vessel; the source gives a geometric amount.
Deionized or distilled water	1 VARIABLE	Add until water stands 5 cm above the soil.

Steam for 1 hour twice at a 24-hour interval, decant and centrifuge, then autoclave aliquots for 20 minutes at 121 C. Concentrations are unspecified by the source.

SAG medium 1 micronutrient solution

Dose: 5 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
ZnSO4 x 7 H2O	1 MG_PER_L
MnSO4 x 4 H2O	2 MG_PER_L
H3BO3	10 MG_PER_L
Co(NO3)2 x 6 H2O	1 MG_PER_L
Na2MoO4 x 2 H2O	1 MG_PER_L
CuSO4 x 5 H2O	0.005 MG_PER_L
FeSO4 x 7 H2O	700 MG_PER_L
EDTA	800 MG_PER_L
Deionized or distilled water	981 ML_PER_L

Stock concentrations are calculated from the source primary-stock volumes into the 1 L applied solution. Prepare as two separately autoclaved solutions and unite after cooling.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	sag-import	Imported from SAG Culture Collection	Source ID: Desmidiacean, PDF URL: http://sagdb.uni-goettingen.de/culture_media/07 Desmidiacean Medium.pdf
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added placeholder ingredients for missing composition
2026-02-03T11:26:43.774559	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.528278Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000199
2026-03-17T19:45:04.454728+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Desmidiacean" to "desmidiacean" for consistent matching
2026-05-16T09:02:56.448544+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.025412+00:00	curate_placeholder_composition.py	REMOVED_PLACEHOLDER_INGREDIENT	Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00	repair_sag_missing_compositions.py	RESTORED_SAG_SOURCE_COMPOSITION	Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM.
2026-09-07T00:00:00-07:00	repair_sag_missing_compositions.py	TAGGED_SAG_RESTORED_COMPOSITION_CURATED	Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags.
2026-09-13T00:00:00-07:00	repair_sag_sources_score10.py	NORMALIZED_SAG_SOURCE_SCORE10	Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring.