dunaliella_acid

CultureMech:000201 algae COMPLEX LIQUID
Original name: Dunaliella_acid

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH range
{'min': 1.5, 'max': 1.8}
Notes
Full recipe available at http://sagdb.uni-goettingen.de/culture_media/18 Dunaliella acid Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/18%20Dunaliella%20acid%20Medium.pdf on 2026-08-25 (SHA-256 b06e06941e5bc5a2d26a89796f770c7215523616d3257d927bf9275723797fc4).

Composition

flowchart LR
    CultureMech_000201["dunaliella_acid"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000201 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_NH4NO3["NH4NO3"]
    CultureMech_000201 --> ing1_NH4NO3
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000201 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000201 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Deionized_or_distilled_water["Deionized or distilled water"]
    CultureMech_000201 --> ing4_Deionized_or_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Deionized_or_distilled_water -.-> CHEBI_15377
    ing5_Vitamin_B12["Vitamin B12"]
    CultureMech_000201 --> ing5_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing5_Vitamin_B12 -.-> CHEBI_176843

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
25.00 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 NH4NO3 0.25 G_PER_L No defensible MediaIngredientMech SSSOM mapping was asserted.
3 K2HPO4
HO4P.2K 174.174 g/mol
0.075 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.012 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 Deionized or distilled water
H2O 18.015 g/mol
990 ML_PER_L CHEBI:15377
water
6 Vitamin B12 0.005 MG_PER_L CHEBI:176843
vitamin B12
 Add in sterile solution after autoclaving.

Preparation

  1. Combine the salts, peat extract, micronutrients, and water.
  2. Adjust to pH 1.5-1.8 with 1 N H2SO4.
  3. Autoclave following the SAG general guidance.
  4. Cool and add vitamin B12 in sterile solution.

Solutions

Peat extract

Dose: 5 ML_PER_L

Stock composition
IngredientConcentrationNotes
Peat soil 1 VARIABLE Fill one third of a vessel; the source gives a geometric amount.
Deionized or distilled water 1 VARIABLE Add until water stands 5 cm above the soil.
Steam for 1 hour twice at a 24-hour interval, decant and centrifuge, then autoclave aliquots for 20 minutes at 121 C. Concentrations are unspecified by the source.

SAG medium 1 micronutrient solution

Dose: 5 ML_PER_L

Stock composition
IngredientConcentrationNotes
ZnSO4 x 7 H2O 1 MG_PER_L
MnSO4 x 4 H2O 2 MG_PER_L
H3BO3 10 MG_PER_L
Co(NO3)2 x 6 H2O 1 MG_PER_L
Na2MoO4 x 2 H2O 1 MG_PER_L
CuSO4 x 5 H2O 0.005 MG_PER_L
FeSO4 x 7 H2O 700 MG_PER_L
EDTA 800 MG_PER_L
Deionized or distilled water 981 ML_PER_L
Stock concentrations are calculated from the source primary-stock volumes into the 1 L applied solution. Prepare as two separately autoclaved solutions and unite after cooling.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 sag-import Imported from SAG Culture Collection Source ID: Dunaliella_acid, PDF URL: http://sagdb.uni-goettingen.de/culture_media/18 Dunaliella acid Medium.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.778508 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.533844Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000201
2026-03-17T19:45:04.460232+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Dunaliella_acid" to "dunaliella_acid" for consistent matching
2026-05-16T09:02:56.460820+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.029398+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_sag_missing_compositions.py RESTORED_SAG_SOURCE_COMPOSITION Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM.
2026-09-07T00:00:00-07:00 repair_sag_missing_compositions.py TAGGED_SAG_RESTORED_COMPOSITION_CURATED Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags.
2026-09-13T00:00:00-07:00 repair_sag_sources_score10.py NORMALIZED_SAG_SOURCE_SCORE10 Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring.