Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at http://sagdb.uni-goettingen.de/culture_media/23 Enriched Seawater Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/23%20Enriched%20Seawater%20Medium.pdf on 2026-08-25 (SHA-256 a74fc20ffe60e1ab58ec5dc02090fdebd5bede978bec0bb2b9ef9236eec10a20).
Composition
flowchart LR
CultureMech_000202["enriched_seawater"]
ing0_Filtered_seawater["Filtered seawater"]
CultureMech_000202 --> ing0_Filtered_seawater
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Filtered seawater | 1000 ML_PER_L |
MICRO:0001773 (source link unavailable)
filtered seawater
|
Preparation
- Add the nutrient, micronutrient, and vitamin stocks to filtered seawater.
- For diatom culture, the source recommends 1 mL/L of a 30 g/L Na2SiO3 x 9 H2O stock.
- Filter sterilize, or acidify slightly and autoclave.
- If autoclaved, add the vitamin working stock after cooling.
Solutions
NaNO3 stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaNO3 | 75 G_PER_L |
NaH2PO4 x H2O stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaH2PO4 x H2O | 5 G_PER_L |
f/2 micronutrient working stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CuSO4 x 5 H2O | 9.8 MG_PER_L | |
| ZnSO4 x 7 H2O | 22 MG_PER_L | |
| CoCl2 x 6 H2O | 10 MG_PER_L | |
| MnCl2 x 4 H2O | 180 MG_PER_L | |
| Na2MoO4 x 2 H2O | 6.3 MG_PER_L | |
| Na2EDTA | 4.36 G_PER_L | The source specifies disodium EDTA without a hydration state. |
| FeCl3 x 6 H2O | 3.15 G_PER_L | |
| Deionized or distilled water | 1 L |
Primary-stock additions were calculated into the 1 L working stock; the primary stock boundary is retained in this derivation note.
f/2 vitamin working stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Vitamin B12 | 1 MG_PER_L | |
| Biotin | 1 MG_PER_L | |
| Thiamine HCl | 200 MG_PER_L | |
| Deionized or distilled water | 1 L |
Primary-stock additions were calculated into the 1 L working stock.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | sag-import | Imported from SAG Culture Collection | Source ID: Enriched_Seawater, PDF URL: http://sagdb.uni-goettingen.de/culture_media/23 Enriched Seawater Medium.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.780490 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.536695Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000202 |
| 2026-03-17T19:45:04.463297+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Enriched_Seawater" to "enriched_seawater" for consistent matching |
| 2026-05-16T09:02:56.527683+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-06T07:56:36.031093+00:00 | curate_placeholder_composition.py | REMOVED_PLACEHOLDER_INGREDIENT | Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175). |
| 2026-08-25T00:00:00-07:00 | repair_sag_missing_compositions.py | RESTORED_SAG_SOURCE_COMPOSITION | Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM. |
| 2026-09-07T00:00:00-07:00 | repair_sag_missing_compositions.py | TAGGED_SAG_RESTORED_COMPOSITION_CURATED | Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags. |
| 2026-09-13T00:00:00-07:00 | repair_sag_sources_score10.py | NORMALIZED_SAG_SOURCE_SCORE10 | Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring. |