note_on_biphasic_soilwater_media_20081216

CultureMech:000205 algae COMPLEX BIPHASIC
Original name: Note_on_biphasic_soilwater_media_20081216

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
Full recipe available at http://sagdb.uni-goettingen.de/culture_media/Note_on_biphasic_soilwater_media_20081216.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/Note_on_biphasic_soilwater_media_20081216.pdf on 2026-08-25 (SHA-256 36370756f412e9d6e84491114f59ec91a7fc7099b1657f23a3fe07a484bfd518).

Composition

flowchart LR
    CultureMech_000205["note_on_biphasic_soilwater_media_20081216"]
    ing0_Garden_soil["Garden soil"]
    CultureMech_000205 --> ing0_Garden_soil
    ing1_Deionized_or_distilled_water["Deionized or distilled water"]
    CultureMech_000205 --> ing1_Deionized_or_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Deionized_or_distilled_water -.-> CHEBI_15377

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Garden soil 1 VARIABLE ENVO:00002263
garden soil
 Place a 1-2 cm soil layer in the bottom of the vessel.
2 Deionized or distilled water
H2O 18.015 g/mol
1 VARIABLE CHEBI:15377
water
 Fill the vessel three-quarters full over the soil layer.

Preparation

  1. Optionally place a small amount of the selected insoluble organic or inorganic supplement in the vessel.
  2. Add 1-2 cm garden soil and overlay with water to three-quarters full.
  3. Steam for 1 hour on two consecutive days; do not autoclave.
  4. Source variants use CaCO3, NH4MgPO4, soaked pea, barley, wheat, sand, loam, or peat.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 sag-import Imported from SAG Culture Collection Source ID: Note_on_biphasic_soilwater_media_20081216, PDF URL: http://sagdb.uni-goettingen.de/culture_media/Note_on_biphasic_soilwater_media_20081216.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.786432 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.544996Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000205
2026-03-17T19:45:04.471332+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Note_on_biphasic_soilwater_media_20081216" to "note_on_biphasic_soilwater_media_20081216" for consistent matching
2026-05-16T09:02:56.814909+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.039239+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_sag_missing_compositions.py RESTORED_SAG_SOURCE_COMPOSITION Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM.
2026-09-07T00:00:00-07:00 repair_sag_missing_compositions.py TAGGED_SAG_RESTORED_COMPOSITION_CURATED Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags.
2026-09-13T00:00:00-07:00 repair_sag_sources_score10.py NORMALIZED_SAG_SOURCE_SCORE10 Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring.