ochromonas

CultureMech:000206 algae COMPLEX LIQUID
Original name: Ochromonas

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
Full recipe available at http://sagdb.uni-goettingen.de/culture_media/10 Ochromonas Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/10%20Ochromonas%20Medium.pdf on 2026-08-25 (SHA-256 8b15d4c6303bc0e583d73e8d1e861c6a63ab5374bcadc58fa57af1bf3e83d63e).

Composition

flowchart LR
    CultureMech_000206["ochromonas"]
    ing0_Deionized_or_distilled_water["Deionized or distilled water"]
    CultureMech_000206 --> ing0_Deionized_or_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Deionized_or_distilled_water -.-> CHEBI_15377

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 Deionized or distilled water
H2O 18.015 g/mol
960 ML_PER_L CHEBI:15377
water

Preparation

  1. Prepare the stock solutions and combine them at the stated amounts.
  2. Autoclave at 121 C for 15 minutes following the SAG general preparation guidance.

Solutions

Glucose stock solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
Glucose 100 G_PER_L

Bacto-tryptone stock solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
Bacto-tryptone 100 G_PER_L

Liver extract stock solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
Liver extract 100 G_PER_L

Yeast extract stock solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
Yeast extract 100 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 sag-import Imported from SAG Culture Collection Source ID: Ochromonas, PDF URL: http://sagdb.uni-goettingen.de/culture_media/10 Ochromonas Medium.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.788375 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.547917Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000206
2026-03-17T19:45:04.474032+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Ochromonas" to "ochromonas" for consistent matching
2026-05-16T09:02:56.830481+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.041151+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_sag_missing_compositions.py RESTORED_SAG_SOURCE_COMPOSITION Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM.
2026-09-07T00:00:00-07:00 repair_sag_missing_compositions.py TAGGED_SAG_RESTORED_COMPOSITION_CURATED Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags.
2026-09-13T00:00:00-07:00 repair_sag_sources_score10.py NORMALIZED_SAG_SOURCE_SCORE10 Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring.