pes

CultureMech:000207 algae COMPLEX LIQUID
Original name: PES

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
Full recipe available at http://sagdb.uni-goettingen.de/culture_media/29 PES Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/29%20PES%20Medium.pdf on 2026-08-25 (SHA-256 951aaf669593437fca489369dda2e8e88737f598c3b984da1185e6b0c52e76f1).

Composition

flowchart LR
    CultureMech_000207["pes"]
    ing0_Pasteurized__filtered_seawater["Pasteurized, filtered seawater"]
    CultureMech_000207 --> ing0_Pasteurized__filtered_seawater

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 Pasteurized, filtered seawater 1000 ML_PER_L ENVO:00002149
sea water

Preparation

  1. Prepare, adjust, dispense, and autoclave the ES-enrichment stock.
  2. Add one 20 mL tube of ES-enrichment to 1000 mL pasteurized, filtered seawater.
  3. Store ES-enrichment stock at 10 C.

Solutions

PES ES-enrichment solution

Dose: 20 ML_PER_L

Stock composition
IngredientConcentrationNotes
NaNO3 3500 MG_PER_L
Na2glycerophosphate x 5 H2O 500 MG_PER_L
Fe(NH4)2(SO4)2 x 6 H2O 175.5 MG_PER_L
Na2EDTA 400 MG_PER_L Calculated as 150 mg/L from the Fe solution plus 250 mg/L from the PII metal solution; hydration state unspecified.
H3BO3 285 MG_PER_L
FeCl3 x 6 H2O 12.25 MG_PER_L
MnSO4 x H2O 41 MG_PER_L
ZnSO4 x 7 H2O 5.5 MG_PER_L
CoSO4 x 7 H2O 1.2 MG_PER_L
Vitamin B12 0.1 MG_PER_L
Thiamine 5 MG_PER_L
Biotin 0.05 MG_PER_L
TRIS 5 G_PER_L No exact MediaIngredientMech SSSOM mapping was available.
Deionized or distilled water 1 L
Source amounts per 100 mL were normalized to the enrichment stock. Fe-solution and PII-solution contributions were calculated into this one LinkML composition level while retaining their origins in notes. Adjust this stock to pH 7.8, dispense 20 mL portions, and autoclave.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 sag-import Imported from SAG Culture Collection Source ID: PES, PDF URL: http://sagdb.uni-goettingen.de/culture_media/29 PES Medium.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.790281 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.550787Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000207
2026-03-17T19:45:04.476599+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PES" to "pes" for consistent matching
2026-05-16T09:02:56.853521+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.042829+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_sag_missing_compositions.py RESTORED_SAG_SOURCE_COMPOSITION Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM.
2026-09-07T00:00:00-07:00 repair_sag_missing_compositions.py TAGGED_SAG_RESTORED_COMPOSITION_CURATED Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags.
2026-09-13T00:00:00-07:00 repair_sag_sources_score10.py NORMALIZED_SAG_SOURCE_SCORE10 Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring.