porphyridium (CultureMech:000210)

Overview

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

Full recipe available at http://sagdb.uni-goettingen.de/culture_media/08 Porphyridium Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/08%20Porphyridium%20Medium.pdf on 2026-08-25 (SHA-256 4af6add4c928721374b38132933f522d01fe62230c2b29747ed59c4291cc69d1).

Composition

flowchart LR
    CultureMech_000210["porphyridium"]
    ing0_Deionized_or_distilled_water["Deionized or distilled water"]
    CultureMech_000210 --> ing0_Deionized_or_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Deionized_or_distilled_water -.-> CHEBI_15377
    ing1_Filtered_seawater["Filtered seawater"]
    CultureMech_000210 --> ing1_Filtered_seawater

Ingredients (2)

#	Ingredient	Concentration	Ontology	Notes
1	Deionized or distilled water H2O 18.015 g/mol	390 ML_PER_L	CHEBI:15377 water
2	Filtered seawater	500 ML_PER_L	MICRO:0001773 (source link unavailable) filtered seawater

Preparation

Prepare the stock solutions and combine them at the stated amounts.
Autoclave at 121 C for 15 minutes following the SAG general preparation guidance.

Solutions

KNO3 stock solution

Dose: 20 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
KNO3	10 G_PER_L

K2HPO4 stock solution

Dose: 20 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
K2HPO4	1 G_PER_L

MgSO4 x 7 H2O stock solution

Dose: 20 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
MgSO4 x 7 H2O	1 G_PER_L

Yeast extract stock solution

Dose: 10 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Yeast extract	100 G_PER_L

Bacto-tryptone stock solution

Dose: 10 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Bacto-tryptone	100 G_PER_L

Soil extract

Dose: 30 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Garden or leaf soil	1 VARIABLE	Fill one third of a vessel; the source gives a geometric amount.
Deionized or distilled water	1 VARIABLE	Add until water stands 5 cm above the soil.

Steam for 1 hour twice at a 24-hour interval, decant and centrifuge, then autoclave aliquots for 20 minutes at 121 C. Concentrations are unspecified by the source.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	sag-import	Imported from SAG Culture Collection	Source ID: Porphyridium, PDF URL: http://sagdb.uni-goettingen.de/culture_media/08 Porphyridium Medium.pdf
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added placeholder ingredients for missing composition
2026-02-03T11:26:43.796115	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.559585Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000210
2026-03-17T19:45:04.484246+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Porphyridium" to "porphyridium" for consistent matching
2026-05-16T09:02:56.875471+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.047164+00:00	curate_placeholder_composition.py	REMOVED_PLACEHOLDER_INGREDIENT	Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00	repair_sag_missing_compositions.py	RESTORED_SAG_SOURCE_COMPOSITION	Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM.
2026-09-07T00:00:00-07:00	repair_sag_missing_compositions.py	TAGGED_SAG_RESTORED_COMPOSITION_CURATED	Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags.
2026-09-13T00:00:00-07:00	repair_sag_sources_score10.py	NORMALIZED_SAG_SOURCE_SCORE10	Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring.