spirulina (CultureMech:000213)

Overview

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

Full recipe available at http://sagdb.uni-goettingen.de/culture_media/02 Spirulina Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/02%20Spirulina%20Medium.pdf on 2026-08-25 (SHA-256 21e6036ced504681ada468b15e96f8e4693e13b87e621bcbfabe44be6a2c9cd8).

Ingredients (0)

No ingredients recorded.

Preparation

Prepare solutions I and II separately.
Autoclave solutions I and II separately.
Cool, then unite equal 500 mL portions.
If required, add sterile vitamin B12 to 5 x 10^-6 g/L; it is optional in the source.

Solutions

Spirulina solution I

Dose: 500 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
NaHCO3	27.22 G_PER_L
Na2CO3	8.06 G_PER_L
K2HPO4	1.00 G_PER_L
Deionized or distilled water	1 L	Prepare solution I to 500 mL.

Composition normalized from the source 500 mL batch.

Spirulina solution II

Dose: 500 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
NaNO3	5.00 G_PER_L
K2SO4	2.00 G_PER_L
NaCl	2.00 G_PER_L
MgSO4 x 7 H2O	0.40 G_PER_L
CaCl2 x 2 H2O	0.08 G_PER_L
FeSO4 x 7 H2O	0.02 G_PER_L
EDTA	0.16 G_PER_L
Deionized or distilled water	1 L	Prepare solution II to 500 mL.

Composition normalized from the source 500 mL batch. Its 5 mL micronutrient addition is modeled as a separate final-recipe stock because all of solution II is used.

SAG medium 1 micronutrient solution

Dose: 5 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
ZnSO4 x 7 H2O	1 MG_PER_L
MnSO4 x 4 H2O	2 MG_PER_L
H3BO3	10 MG_PER_L
Co(NO3)2 x 6 H2O	1 MG_PER_L
Na2MoO4 x 2 H2O	1 MG_PER_L
CuSO4 x 5 H2O	0.005 MG_PER_L
FeSO4 x 7 H2O	700 MG_PER_L
EDTA	800 MG_PER_L
Deionized or distilled water	981 ML_PER_L

Stock concentrations are calculated from the source primary-stock volumes into the 1 L applied solution. Prepare as two separately autoclaved solutions and unite after cooling.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	sag-import	Imported from SAG Culture Collection	Source ID: Spirulina, PDF URL: http://sagdb.uni-goettingen.de/culture_media/02 Spirulina Medium.pdf
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added placeholder ingredients for missing composition
2026-02-03T11:26:43.802317	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.568926Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000213
2026-03-17T19:45:04.492636+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Spirulina" to "spirulina" for consistent matching
2026-05-16T09:02:57.041652+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.060542+00:00	curate_placeholder_composition.py	REMOVED_PLACEHOLDER_INGREDIENT	Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00	repair_sag_missing_compositions.py	RESTORED_SAG_SOURCE_COMPOSITION	Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM.
2026-09-07T00:00:00-07:00	repair_sag_missing_compositions.py	TAGGED_SAG_RESTORED_COMPOSITION_CURATED	Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags.
2026-09-13T00:00:00-07:00	repair_sag_sources_score10.py	NORMALIZED_SAG_SOURCE_SCORE10	Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring.