unicellular_green_algae

CultureMech:000214 algae COMPLEX LIQUID
Original name: Unicellular_Green_Algae

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
Full recipe available at http://sagdb.uni-goettingen.de/culture_media/12 Unicellular Green Algae Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/12%20Unicellular%20Green%20Algae%20Medium.pdf on 2026-08-25 (SHA-256 7c767329e2b64fe81d9fe55f28bfcef8245a5ba3b83397824eda9bbcc86164f4).

Composition

flowchart LR
    CultureMech_000214["unicellular_green_algae"]
    ing0_KNO3["KNO3"]
    CultureMech_000214 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
    CultureMech_000214 --> ing1_NaH2PO4_x_H2O
    CHEBI_37585["CHEBI:37585"]
    ing1_NaH2PO4_x_H2O -.-> CHEBI_37585
    ing2_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_000214 --> ing2_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing2_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000214 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000214 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Deionized_or_distilled_water["Deionized or distilled water"]
    CultureMech_000214 --> ing5_Deionized_or_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Deionized_or_distilled_water -.-> CHEBI_15377

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 KNO3
K.NO3 101.102 g/mol
1011.1 MG_PER_L CHEBI:63043
potassium nitrate
2 NaH2PO4 x H2O
H2O4P.Na 119.976 g/mol
621 MG_PER_L CHEBI:37585
sodium dihydrogenphosphate
3 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
89 MG_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
246.5 MG_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
14.7 MG_PER_L CHEBI:86158
calcium chloride dihydrate
6 Deionized or distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
 Make the final medium to 1 L.

Preparation

  1. Prepare the stock solutions and combine them at the stated amounts.
  2. Autoclave at 121 C for 15 minutes following the SAG general preparation guidance.

Solutions

Fe-EDTA complex

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 6.9 G_PER_L
Disodium EDTA 9.3 G_PER_L The source specifies disodium EDTA but no hydration state.
Deionized or distilled water 1 L Prepare the stock to 100 mL.
Boil briefly, cool, and make to volume. The medium table prints 6.95 mg/L FeSO4 x 7 H2O; the stock recipe calculates to 6.9 mg/L.

Unicellular green algae micronutrient solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
H3BO3 61.0 MG_PER_L
MnSO4 x H2O 169.0 MG_PER_L
ZnSO4 x 7 H2O 287.0 MG_PER_L
CuSO4 x 5 H2O 2.5 MG_PER_L
(NH4)6Mo7O24 x 4 H2O 12.5 MG_PER_L
Deionized or distilled water 1 L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 sag-import Imported from SAG Culture Collection Source ID: Unicellular_Green_Algae, PDF URL: http://sagdb.uni-goettingen.de/culture_media/12 Unicellular Green Algae Medium.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.804600 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.571806Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000214
2026-03-17T19:45:04.495425+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Unicellular_Green_Algae" to "unicellular_green_algae" for consistent matching
2026-05-16T09:02:57.089045+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.062044+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_sag_missing_compositions.py RESTORED_SAG_SOURCE_COMPOSITION Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM.
2026-09-07T00:00:00-07:00 repair_sag_missing_compositions.py TAGGED_SAG_RESTORED_COMPOSITION_CURATED Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags.
2026-09-13T00:00:00-07:00 repair_sag_sources_score10.py NORMALIZED_SAG_SOURCE_SCORE10 Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring.