volvox

CultureMech:000215 algae COMPLEX LIQUID
Original name: Volvox

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH range
{'notes': 'The source gives two alternatives: pH 7.0 or pH 8.0.'}
Notes
Full recipe available at http://sagdb.uni-goettingen.de/culture_media/22 Volvox Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/22%20Volvox%20Medium.pdf on 2026-08-25 (SHA-256 78a992920c3a2d858a65417013c16b8a3fd4a0b5642b5dd3b417f27046e40a02).

Composition

flowchart LR
    CultureMech_000215["volvox"]
    ing0_Na2glycerophosphate_x_5_H2O["Na2glycerophosphate x 5 H2O"]
    CultureMech_000215 --> ing0_Na2glycerophosphate_x_5_H2O
    CHEBI_15978["CHEBI:15978"]
    ing0_Na2glycerophosphate_x_5_H2O -.-> CHEBI_15978
    ing1_Glycyl_glycine["Glycyl-glycine"]
    CultureMech_000215 --> ing1_Glycyl_glycine
    CHEBI_17201["CHEBI:17201"]
    ing1_Glycyl_glycine -.-> CHEBI_17201
    ing2_Deionized_or_distilled_water["Deionized or distilled water"]
    CultureMech_000215 --> ing2_Deionized_or_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Deionized_or_distilled_water -.-> CHEBI_15377
    ing3_Vitamin_B12["Vitamin B12"]
    CultureMech_000215 --> ing3_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing3_Vitamin_B12 -.-> CHEBI_176843
    ing4_Thiamine["Thiamine"]
    CultureMech_000215 --> ing4_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing4_Thiamine -.-> CHEBI_18385

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Na2glycerophosphate x 5 H2O
C3H9O6P 172.073 g/mol
60 MG_PER_L CHEBI:15978
sn-glycerol 3-phosphate
2 Glycyl-glycine
C4H8N2O3 132.119 g/mol
500 MG_PER_L CHEBI:17201
glycylglycine
3 Deionized or distilled water
H2O 18.015 g/mol
964 ML_PER_L CHEBI:15377
water
4 Vitamin B12 0.005 MG_PER_L CHEBI:176843
vitamin B12
 Add in sterile solution after cooling.
5 Thiamine
C12H17N4OS 265.362 g/mol
0.5 MG_PER_L CHEBI:18385
thiamine(1+)
 Add in sterile solution after autoclaving.

Preparation

  1. Combine the stock solutions, direct ingredients, and water.
  2. Adjust to pH 7.0 or, for the source alternative, pH 8.0 with 1 N NaOH.
  3. Autoclave following the SAG general guidance.
  4. Cool and add sterile vitamin B12 and vitamin B1 solutions.

Solutions

Ca(NO3)2 x 4 H2O stock solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
Ca(NO3)2 x 4 H2O 118 G_PER_L

MgSO4 x 7 H2O stock solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
MgSO4 x 7 H2O 40 G_PER_L

KCl stock solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
KCl 50 G_PER_L

Volvox micronutrient solution

Dose: 6 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2EDTA 750 MG_PER_L The source specifies disodium EDTA without a hydration state.
FeCl3 x 6 H2O 97 MG_PER_L
MnCl2 x 4 H2O 41 MG_PER_L
ZnCl2 5 MG_PER_L
CoCl2 x 6 H2O 2 MG_PER_L
Na2MoO4 x 2 H2O 4 MG_PER_L
Deionized or distilled water 1 L
Add salts in the order printed by the source.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 sag-import Imported from SAG Culture Collection Source ID: Volvox, PDF URL: http://sagdb.uni-goettingen.de/culture_media/22 Volvox Medium.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.806716 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.574598Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000215
2026-03-17T19:45:04.498164+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Volvox" to "volvox" for consistent matching
2026-05-16T09:02:57.097240+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.063531+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_sag_missing_compositions.py RESTORED_SAG_SOURCE_COMPOSITION Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM.
2026-09-07T00:00:00-07:00 repair_sag_missing_compositions.py TAGGED_SAG_RESTORED_COMPOSITION_CURATED Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags.
2026-09-13T00:00:00-07:00 repair_sag_sources_score10.py NORMALIZED_SAG_SOURCE_SCORE10 Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring.