Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- pH range
- {'min': 5.4, 'max': 6.0}
- Notes
- Full recipe available at http://sagdb.uni-goettingen.de/culture_media/28 WEES Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/28%20WEES%20Medium.pdf on 2026-08-25 (SHA-256 2ac8f35dfedff05a1d56b0b5e8d48a56e64c681ad47efb4aabe11b2b547ea898).
Composition
flowchart LR
CultureMech_000216["wees"]
ing0_Deionized_or_distilled_water["Deionized or distilled water"]
CultureMech_000216 --> ing0_Deionized_or_distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Deionized_or_distilled_water -.-> CHEBI_15377
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Deionized or distilled water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Prepare the final formulation per litre of water as printed. |
Preparation
- Add each stock and the soil extract at the stated amount.
- Adjust to pH 5.4-6.0 with 0.1 N HCl.
- Autoclave at 121 C for 30 minutes.
Solutions
KNO3 stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| KNO3 | 100 G_PER_L |
MgSO4 x 7 H2O stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgSO4 x 7 H2O | 20 G_PER_L |
(NH4)2HPO4 stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| (NH4)2HPO4 | 20 G_PER_L |
Saturated CaSO4 solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CaSO4 | 1 VARIABLE | Prepare a saturated solution; the source gives no mass concentration. |
WEES PII metal mix without Fe
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| EDTA | 3 G_PER_L | |
| H3BO3 | 1.14 G_PER_L | |
| MnCl2 x 4 H2O | 144 MG_PER_L | |
| ZnSO4 x 7 H2O | 21 MG_PER_L | |
| CoCl2 x 6 H2O | 4 MG_PER_L | |
| Deionized or distilled water | 1 L |
Source amounts per 100 mL were normalized to concentrations per litre.
WEES Fe-EDTA solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| EDTA | 5.2 G_PER_L | |
| FeSO4 x 7 H2O | 5 G_PER_L | |
| 1 N KOH | 54 ML_PER_L | Equivalent to 5.4 mL per source 100 mL stock batch. |
| Deionized or distilled water | 1 L |
WEES vitamin stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Vitamin B12 | 0.2 MG_PER_L | |
| Biotin | 1.0 MG_PER_L | |
| Thiamine HCl | 100 MG_PER_L | |
| Nicotinamide | 0.1 MG_PER_L | |
| Deionized or distilled water | 1 L |
WEES soil extract
Dose: 100 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Garden soil | 100 G_PER_L | |
| Deionized or distilled water | 1 L |
Boil 10 g soil with 125 mL water for 5 minutes, filter and centrifuge, then make the extract to 100 mL.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | sag-import | Imported from SAG Culture Collection | Source ID: WEES, PDF URL: http://sagdb.uni-goettingen.de/culture_media/28 WEES Medium.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.808743 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.577684Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000216 |
| 2026-03-17T19:45:04.500829+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "WEES" to "wees" for consistent matching |
| 2026-05-16T09:02:57.131396+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-06T07:56:36.064916+00:00 | curate_placeholder_composition.py | REMOVED_PLACEHOLDER_INGREDIENT | Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175). |
| 2026-08-25T00:00:00-07:00 | repair_sag_missing_compositions.py | RESTORED_SAG_SOURCE_COMPOSITION | Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM. |
| 2026-09-07T00:00:00-07:00 | repair_sag_missing_compositions.py | TAGGED_SAG_RESTORED_COMPOSITION_CURATED | Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags. |
| 2026-09-13T00:00:00-07:00 | repair_sag_sources_score10.py | NORMALIZED_SAG_SOURCE_SCORE10 | Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring. |