Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at http://sagdb.uni-goettingen.de/culture_media/24 WC Medium.pdf Composition verified against https://sagdb.uni-goettingen.de/culture_media/24%20WC%20Medium.pdf on 2026-08-25 (SHA-256 7c53c28713fc1b1b95f6dcc518e3b9e1324a25ee6ccf7a4064a2cd369f7dcaf7).
Composition
flowchart LR
CultureMech_000217["woods_hole_mbl_medium"]
ing0_TES["TES"]
CultureMech_000217 --> ing0_TES
CHEBI_39035["CHEBI:39035"]
ing0_TES -.-> CHEBI_39035
ing1_Deionized_or_distilled_water["Deionized or distilled water"]
CultureMech_000217 --> ing1_Deionized_or_distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Deionized_or_distilled_water -.-> CHEBI_15377
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | TES | 0.115 G_PER_L |
CHEBI:39035
TES
|
|
| 2 |
Deionized or distilled water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Preparation
- Prepare the stock solutions and combine them at the stated amounts.
- Autoclave at 121 C for 15 minutes following the SAG general preparation guidance.
Solutions
CaCl2 x 2 H2O stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CaCl2 x 2 H2O | 36.8 G_PER_L |
MgSO4 x 7 H2O stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgSO4 x 7 H2O | 37 G_PER_L |
NaHCO3 stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaHCO3 | 12.6 G_PER_L |
The PDF prints 'NaH CO3'; interpreted as sodium hydrogencarbonate.
K2HPO4 x 3 H2O stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| K2HPO4 x 3 H2O | 11.4 G_PER_L |
NaNO3 stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaNO3 | 85 G_PER_L |
Na2SiO3 x 9 H2O stock solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2SiO3 x 9 H2O | 21.2 G_PER_L |
WC micronutrient solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2EDTA | 4.36 G_PER_L | Disodium EDTA; hydration state unspecified. |
| FeCl3 x 6 H2O | 3.15 G_PER_L | |
| CuSO4 x 5 H2O | 0.01 G_PER_L | |
| ZnSO4 x 7 H2O | 0.022 G_PER_L | |
| CoCl2 x 6 H2O | 0.01 G_PER_L | |
| MnCl2 x 4 H2O | 0.18 G_PER_L | |
| Na2MoO4 x 2 H2O | 0.006 G_PER_L | |
| H3BO3 | 1 G_PER_L | |
| Deionized or distilled water | 1 L |
WC vitamin solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Thiamine HCl | 0.1 G_PER_L | |
| Biotin | 0.0005 G_PER_L | |
| Deionized or distilled water | 1 L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | sag-import | Imported from SAG Culture Collection | Source ID: WC, PDF URL: http://sagdb.uni-goettingen.de/culture_media/24 WC Medium.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.810648 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.580650Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000217 |
| 2026-03-17T19:45:04.503613+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Woods Hole MBL Medium" to "woods_hole_mbl_medium" for consistent matching |
| 2026-05-16T09:02:57.139767+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-06T07:56:36.066303+00:00 | curate_placeholder_composition.py | REMOVED_PLACEHOLDER_INGREDIENT | Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175). |
| 2026-08-25T00:00:00-07:00 | repair_sag_missing_compositions.py | RESTORED_SAG_SOURCE_COMPOSITION | Restored the formulation from the reviewed SAG PDF, preserving final-medium and stock-solution boundaries. Calculated stock concentrations are documented in-place. Ingredient identities were selected from the MediaIngredientMech SSSOM. |
| 2026-09-07T00:00:00-07:00 | repair_sag_missing_compositions.py | TAGGED_SAG_RESTORED_COMPOSITION_CURATED | Marked the reviewed SAG source formulation as curated and ontology-mapped after the original restoration left only the ingredient identities and did not add the scoring flags. |
| 2026-09-13T00:00:00-07:00 | repair_sag_sources_score10.py | NORMALIZED_SAG_SOURCE_SCORE10 | Copied the SAG source identity from references and the original sag-import event into the structured sources slot recognized by review scoring. |