snow_algae_medium

CultureMech:000220 algae COMPLEX LIQUID
Original name: Snow Algae Medium

Overview

External ontology
UTEX:snow-algae-medium (source link unavailable) — Snow Algae Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH range
{'min': 5.1, 'max': 5.3}
Notes
UTEX prepares Snow Algae Medium at pH 5.1-5.3 from a defined freshwater salt base with FE EDTA and Minor Nutrients added at source-stated volumes.

Composition

flowchart LR
    CultureMech_000220["snow_algae_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000220 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000220 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_NaNO3["NaNO3"]
    CultureMech_000220 --> ing2_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing2_NaNO3 -.-> CHEBI_63005
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000220 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_000220 --> ing4_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing4_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000220 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_Na2SiO3_x_9_H2O["Na2SiO3 x 9 H2O"]
    CultureMech_000220 --> ing6_Na2SiO3_x_9_H2O
    CHEBI_132108["CHEBI:132108"]
    ing6_Na2SiO3_x_9_H2O -.-> CHEBI_132108
    ing7_K2HPO4["K2HPO4"]
    CultureMech_000220 --> ing7_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing7_K2HPO4 -.-> CHEBI_131527
    ing8_KH2PO4["KH2PO4"]
    CultureMech_000220 --> ing8_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing8_KH2PO4 -.-> CHEBI_63036

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts Snow Algae Medium with approximately 500 mL dH2O.
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
Sigma C-3881
0.03 MILLIMOLAR CHEBI:86158
calcium chloride dihydrate
 UTEX adds 1 mL/L of a 0.5 g/100 mL dH2O stock, for 0.03 mM final.
3 NaNO3
NO3.Na 84.994 g/mol
Fisher BP360-500
0.29 MILLIMOLAR CHEBI:63005
sodium nitrate
 UTEX adds 1 mL/L of a 2.5 g/100 mL dH2O stock, for 0.29 mM final.
4 NH4Cl
Cl.H4N 53.492 g/mol
Fisher A 649-500
0.093 MILLIMOLAR CHEBI:31206
ammonium chloride
 UTEX adds 1 mL/L of a 0.5 g/100 mL dH2O stock, for 0.093 mM final.
5 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
Mallinckroft 4300
0.023 MILLIMOLAR CHEBI:32583
calcium sulfate dihydrate
 UTEX adds 1 mL/L of a 0.4 g/100 mL dH2O stock, for 0.023 mM final.
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
Sigma 230391
0.02 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 UTEX adds 1 mL/L of a 0.5 g/100 mL dH2O stock, for 0.02 mM final.
7 Na2SiO3 x 9 H2O
9H2O.2Na.O3Si 284.198 g/mol
Sigma 307815
0.007 MILLIMOLAR CHEBI:132108
sodium silicate nonahydrate
 UTEX adds 1 mL/L of a 0.2 g/100 mL dH2O stock, for 0.007 mM final.
8 K2HPO4
HO4P.2K 174.174 g/mol
Sigma P 3786
0.4 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
 UTEX adds 2 mL of a 3.48 g/100 mL dH2O stock per liter, for 0.4 mM final.
9 KH2PO4
H2O4P.K 136.084 g/mol
Sigma P 0662
0.02 MOLAR CHEBI:63036
potassium dihydrogen phosphate
 UTEX adds 98 mL of a 2.72 g/100 mL dH2O stock per liter, for 0.02 M final.

Preparation

  1. To approximately 500 mL dH2O, add each component in the UTEX order while stirring continuously.
  2. Bring the total volume to 1 L with dH2O; pH is 5.1-5.3.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

Solutions

FE EDTA

Dose: 250 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings the combined Na2EDTA and iron solution to 1 L with dH2O.
Na2EDTA x 2 H2O 0.66 G_PER_L UTEX lists 0.66 g/L Na2EDTA x 2 H2O, for 1.7 mM final in the stock.
Fe(NH4)2(SO4)2 x 6 H2O 0.7 G_PER_L UTEX lists 0.7 g/L Fe(NH4)2(SO4)2 x 6 H2O, for 1.7 mM final in the stock.
UTEX adds 250 mL FE EDTA per liter of Snow Algae Medium; final concentrations are listed for the FE EDTA stock.

Minor Nutrients

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX starts Minor Nutrients with approximately 950 mL dH2O.
H3BO3 0.248 G_PER_L UTEX lists 0.248 g/L H3BO3, for 1.01 mM final in the stock.
MnSO4 x H2O 0.184 G_PER_L UTEX lists 0.184 g/L MnSO4 x H2O, for 1.01 mM final in the stock.
ZnSO4 x 7 H2O 0.023 G_PER_L UTEX lists 0.023 g/L ZnSO4 x 7 H2O, for 0.08 mM final in the stock.
(NH4)6Mo7O24 x 4 H2O 0.007 G_PER_L UTEX lists 0.007 g/L (NH4)6Mo7O24 x 4 H2O, for 0.01 mM final in the stock.
Co(NO3)2 x 6 H2O 0.012 G_PER_L UTEX lists 0.012 g/L Co(NO3)2 x 6 H2O, for 0.04 mM final in the stock.
KBr 0.015 G_PER_L UTEX lists 0.015 g/L KBr, for 0.13 mM final in the stock.
NiSO4(NH4)SO4 x 6 H2O 0.016 G_PER_L UTEX lists 0.016 g/L NiSO4(NH4)SO4 x 6 H2O, for 0.09 mM final in the stock; no exact CHEBI grounding is asserted for the source formula.
VOSO4 x 2 H2O 0.0016 G_PER_L UTEX lists 0.0016 g/L VOSO4 x 2 H2O, for 0.01 mM final in the stock.
Al2(SO4)3K2SO4 x 24 H2O 0.0376 G_PER_L UTEX lists 0.0376 g/L Al2(SO4)3.K2SO4.24H2O, for 0.04 mM final in the stock.
UTEX adds 10 mL Minor Nutrients per liter of Snow Algae Medium; final concentrations are listed for the Minor Nutrients stock.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: snow-algae-medium, URL: https://utex.org/products/snow-algae-medium
2026-03-13T06:18:53.593701Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000220
2026-03-17T19:45:04.514633+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Snow Algae Medium" to "snow_algae_medium" for consistent matching
2026-05-16T09:02:56.104813+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.317655+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.959798+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 10 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_n20_snow_score35.py RESOLVED_UTEX_N20_SNOW_SCORE35 UTEX prepares Snow Algae Medium at pH 5.1-5.3 from a defined freshwater salt base with FE EDTA and Minor Nutrients added at source-stated volumes.