soilwater_pea_medium

CultureMech:000229 algae COMPLEX LIQUID
Original name: Soilwater: PEA Medium

Overview

External ontology
UTEX:soilwater-pea-medium (source link unavailable) — Soilwater: PEA Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares Soilwater: PEA Medium by steaming Green House Soil, one pea, CaCO3, and dH2O.

Composition

flowchart LR
    CultureMech_000229["soilwater_pea_medium"]
    ing0_Green_House_Soil["Green House Soil"]
    CultureMech_000229 --> ing0_Green_House_Soil
    ing1_Pea["Pea"]
    CultureMech_000229 --> ing1_Pea
    ing2_CaCO3["CaCO3"]
    CultureMech_000229 --> ing2_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing2_CaCO3 -.-> CHEBI_3311
    ing3_dH2O["dH2O"]
    CultureMech_000229 --> ing3_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing3_dH2O -.-> CHEBI_15377

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Green House Soil 1 tsp per 200 mL VARIABLE UTEX lists 1 tsp per 200 mL Green House Soil.
2 Pea 1 per 200 mL VARIABLE UTEX lists one pea per 200 mL, added after dH2O.
3 CaCO3
CO3.Ca 100.086 g/mol
Fisher C 64
0.05 MILLIMOLAR CHEBI:3311
calcium carbonate
 UTEX lists 1 mg CaCO3 per 200 mL final medium; final 0.05 mM.
4 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 Converted from 200 mL dH2O per 200 mL final medium.

Preparation

  1. Combine Green House Soil, one pea, CaCO3, and dH2O.
  2. Cover and steam for 2 consecutive days, 3 hours on each day.
  3. Refrigerate for at least 24 hours, then bring to room temperature before use.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: soilwater-pea-medium, URL: https://utex.org/products/soilwater-pea-medium
2026-03-13T06:18:53.639138Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000229
2026-03-17T19:45:04.552234+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Soilwater: PEA Medium" to "soilwater_pea_medium" for consistent matching
2026-05-16T09:02:56.129585+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.343958+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.994320+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 4 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_soilwater_score35.py RESOLVED_UTEX_SOILWATER_SCORE35 UTEX prepares Soilwater: PEA Medium by steaming Green House Soil, one pea, CaCO3, and dH2O.