Overview
- External ontology
- UTEX:spirulina-medium (source link unavailable) — Spirulina Medium
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- UTEX lists Spirulina Medium as an alkaline modification of SAG medium prepared by sterilizing separate carbonate/phosphate and nitrate/salt solutions before mixing.
Ingredients (0)
No ingredients recorded.
Preparation
- Prepare Spir solution 1 by adding NaHCO3, Na2CO3, and K2HPO4 to approximately 450 mL dH2O while stirring.
- Bring Spir solution 1 to 500 mL with dH2O.
- Autoclave Spir solution 1.
- Prepare Spir solution 2 by adding NaNO3, K2SO4, NaCl, MgSO4 x 7 H2O, CaCl2 x 2 H2O, P-IV Metal Solution, Chu Micronutrient Solution, and Vitamin B12 to approximately 450 mL dH2O while stirring.
- Bring Spir solution 2 to 500 mL with dH2O.
- Autoclave Spir solution 2.
- Transfer Spir solution 2 into Spir solution 1 and swirl thoroughly.
- Store at refrigerator temperature.
Solutions
Spir solution 1
Dose: 500 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | 1000 ML_PER_L | UTEX brings Spir solution 1 to 500 mL with dH2O. |
| NaHCO3 | 27.22 G_PER_L | Converted from 13.61 g/500 mL in the UTEX Spir solution 1 recipe. |
| Na2CO3 | 8.06 G_PER_L | Converted from 4.03 g/500 mL in the UTEX Spir solution 1 recipe. |
| K2HPO4 | 1 G_PER_L | Converted from 0.5 g/500 mL in the UTEX Spir solution 1 recipe. |
UTEX adds 500 mL Spir solution 1 per 1 L liquid Spirulina Medium.
Spir solution 2
Dose: 500 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | 1000 ML_PER_L | UTEX brings Spir solution 2 to 500 mL with dH2O. |
| NaNO3 | 5 G_PER_L | Converted from 2.5 g/500 mL in the UTEX Spir solution 2 recipe. |
| K2SO4 | 2 G_PER_L | Converted from 1 g/500 mL in the UTEX Spir solution 2 recipe. |
| NaCl | 2 G_PER_L | Converted from 1 g/500 mL in the UTEX Spir solution 2 recipe. |
| MgSO4 x 7 H2O | 0.4 G_PER_L | Converted from 0.2 g/500 mL in the UTEX Spir solution 2 recipe. |
| CaCl2 x 2 H2O | 0.08 G_PER_L | Converted from 0.04 g/500 mL in the UTEX Spir solution 2 recipe. |
UTEX adds 500 mL Spir solution 2 per 1 L liquid Spirulina Medium.
P-IV Metal Solution
Dose: 6 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | 1000 ML_PER_L | UTEX brings P-IV Metal Solution to 1 L with dH2O. |
| Na2EDTA x 2 H2O | 0.75 G_PER_L | |
| FeCl3 x 6 H2O | 0.097 G_PER_L | |
| MnCl2 x 4 H2O | 0.041 G_PER_L | |
| ZnCl2 | 0.005 G_PER_L | |
| CoCl2 x 6 H2O | 0.002 G_PER_L | |
| Na2MoO4 x 2 H2O | 0.004 G_PER_L |
UTEX adds 6 mL P-IV Metal Solution to the 500 mL Spir solution 2 stock.
Chu Micronutrient Solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | 1000 ML_PER_L | UTEX brings Chu Micronutrient Solution to 1 L with dH2O. |
| CuSO4 x 5 H2O | 0.02 G_PER_L | |
| ZnSO4 x 7 H2O | 0.044 G_PER_L | |
| CoCl2 x 6 H2O | 0.02 G_PER_L | |
| MnCl2 x 4 H2O | 0.012 G_PER_L | |
| Na2MoO4 x 2 H2O | 0.012 G_PER_L | |
| H3BO3 | 0.62 G_PER_L | |
| Na2EDTA x 2 H2O | 0.05 G_PER_L |
UTEX adds 1 mL Chu Micronutrient Solution to the 500 mL Spir solution 2 stock.
Vitamin B12
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | 1000 ML_PER_L | UTEX prepares the Vitamin B12 stock in 200 mL HEPES buffer. |
| HEPES buffer pH 7.8 | 12 G_PER_L | Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock. |
| Vitamin B12 | 0.135 G_PER_L | Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock. |
UTEX adds 1 mL Vitamin B12 stock to the 500 mL Spir solution 2 stock.
Target organisms and growth evidence
These are curated observations and conditions for named organisms; they do not imply universal growth suitability.
- Preferred term
- Arthrospira platensis
- Term
- Id
- NCBITaxon:118562
- Label
- Limnospira platensis
- Growth metrics
- Measurement conditions
- Conventional Spirulina medium control compared with effluent-based medium during Arthrospira platensis cultivation.
- Evidence
- Reference
- PMID:28025700
- Supports
- SUPPORT
- Snippet
- Equal biomass production was observed until late exponential phase and no significant differences could be observed between the treatments in protein amount, amino acid composition, and total lipid concentration.
- Explanation
- Literature evidence reports biomass production for Arthrospira platensis in conventional Spirulina medium and effluent-based medium.
- Is max attainment
- False
Recipe variants
- Name
- biogas_effluent_based_arthrospira_platensis
- Description
- Effluent-based cultivation medium compared with conventional Spirulina medium for Arthrospira platensis biomass production.
- Modifications
- Use effluent from biogas production as the nutrient source.
- Compare biomass composition and pigment production with conventional Spirulina medium.
- Purpose
- Capture a study-specific Spirulina medium alternative using biogas effluent as recycled nutrients.
- Evidence
- Reference
- PMID:28025700
- Supports
- SUPPORT
- Snippet
- The effluent from the biogas process was tested as a nutrient source during cultivation of the protein-rich and edible microalgae Spirulina (Arthrospira platensis) and compared with conventional Spirulina medium.
- Explanation
- Literature evidence describes an effluent-based medium variant compared with the conventional Spirulina parent medium.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28 | utex-import | Imported from UTEX Culture Collection | Source ID: spirulina-medium, URL: https://utex.org/products/spirulina-medium |
| 2026-03-13T06:18:53.653526Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000232 |
| 2026-03-17T19:45:04.564682+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Spirulina Medium" to "spirulina_medium" for consistent matching |
| 2026-08-06T00:58:02.521300+00:00 | curate_organism_culture_type.py | SET_ORGANISM_CULTURE_TYPE | Inferred isolate: 1 specific strain(s) named, no community signal (#142) |
| 2026-08-30T00:58:31.015871+00:00 | repair_utex_ingredients.py | REPAIRED_UTEX_COMPOSITION | Restored 2 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779. |
| 2026-09-05T00:00:00-07:00 | repair_utex_bg11_spirulina.py | RESOLVED_UTEX_BG11_SPIRULINA_GRAPH | UTEX lists Spirulina Medium as an alkaline modification of SAG medium prepared by sterilizing separate carbonate/phosphate and nitrate/salt solutions before mixing. |