tap_medium

CultureMech:000233 algae COMPLEX LIQUID
Original name: TAP Medium

Overview

External ontology
UTEX:tap-medium (source link unavailable) — TAP Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares TAP Medium from Beijerinck's Solution, Phosphate Buffer Stock Solution, Hunter's Trace Stock Solution, and Tris Acetate Stock Solution added at source-stated volumes.

Composition

flowchart LR
    CultureMech_000233["tap_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000233 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts the 2 L TAP Medium recipe with approximately 1600 mL dH2O.

Preparation

  1. To approximately 1600 mL dH2O, add Beijerinck's Solution, Phosphate Buffer Stock Solution, Hunter's Trace Stock Solution, and Tris Acetate Stock Solution in the UTEX order.
  2. Bring total volume to 2 L with dH2O.
  3. Cover and autoclave the medium.
  4. Cool the autoclaved medium.
  5. Store at refrigerator temperature.

Solutions

Beijerinck's Solution

Dose: 50 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings Beijerinck's Solution to 1 L with dH2O.
NH4Cl 8 G_PER_L UTEX lists 8 g/L NH4Cl in the stock, for 7.5 mM after 50 mL/L dilution into TAP Medium.
CaCl2 x 2 H2O 1 G_PER_L UTEX lists 1 g/L CaCl2 x 2 H2O in the stock, for 0.35 mM after 50 mL/L dilution into TAP Medium.
MgSO4 x 7 H2O 2 G_PER_L UTEX lists 2 g/L MgSO4 x 7 H2O in the stock, for 0.4 mM after 50 mL/L dilution into TAP Medium.
UTEX adds 100 mL Beijerinck's Solution per 2 L of TAP Medium.

Phosphate Buffer Stock Solution

Dose: 8.5 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings Phosphate Buffer Stock Solution to 1 L with dH2O.
Na2HPO4 11.62 G_PER_L UTEX lists 11.62 g/L Na2HPO4 in the stock, for 0.69 mM after 8.5 mL/L dilution into TAP Medium.
KH2PO4 7.26 G_PER_L UTEX lists 7.26 g/L KH2PO4 in the stock, for 0.45 mM after 8.5 mL/L dilution into TAP Medium.
UTEX adds 17 mL Phosphate Buffer Stock Solution per 2 L of TAP Medium.

Hunter's Trace Stock Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings Hunter's Trace Stock Solution to 1 L with dH2O.
Na2EDTA x 2 H2O 50 G_PER_L UTEX lists 50 g/L Na2EDTA x 2 H2O in the stock, for 0.134 mM after 1 mL/L dilution into TAP Medium.
ZnSO4 x 7 H2O 22 G_PER_L UTEX lists 22 g/L ZnSO4 x 7 H2O in the stock, for 0.077 mM after 1 mL/L dilution into TAP Medium.
H3BO3 11.4 G_PER_L UTEX lists 11.4 g/L H3BO3 in the stock, for 0.184 mM after 1 mL/L dilution into TAP Medium.
MnCl2 x 4 H2O 5.1 G_PER_L UTEX lists 5.1 g/L MnCl2 x 4 H2O in the stock, for 0.026 mM after 1 mL/L dilution into TAP Medium.
FeSO4 x 7 H2O 5 G_PER_L UTEX lists 5 g/L FeSO4 x 7 H2O in the stock, for 0.018 mM after 1 mL/L dilution into TAP Medium.
CoCl2 x 6 H2O 1.6 G_PER_L UTEX lists 1.6 g/L CoCl2 x 6 H2O in the stock, for 0.007 mM after 1 mL/L dilution into TAP Medium.
CuSO4 x 5 H2O 1.16 G_PER_L UTEX lists 1.16 g/L CuSO4 x 5 H2O in the stock, for 0.005 mM after 1 mL/L dilution into TAP Medium.
(NH4)6Mo7O24 x 4 H2O 1.1 G_PER_L UTEX lists 1.1 g/L (NH4)6Mo7O24 x 4 H2O in the stock, for 0.0008 mM after 1 mL/L dilution into TAP Medium.
UTEX adds 2 mL Hunter's Trace Stock Solution per 2 L of TAP Medium.

Tris Acetate Stock Solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings Tris Acetate Stock Solution to 500 mL with dH2O.
Trisma Base 242 G_PER_L Converted from 121 g Trisma Base per 500 mL in the UTEX stock, for 0.02 M after 10 mL/L dilution into TAP Medium.
Glacial Acetic Acid 100 ML_PER_L Converted from 50 mL Glacial Acetic Acid per 500 mL in the UTEX stock, for 0.017 M after 10 mL/L dilution into TAP Medium.
UTEX adds 20 mL Tris Acetate Stock Solution per 2 L of TAP Medium.

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

Recipe variants

Provenance

TimestampCuratorActionNotes
2026-01-28 utex-import Imported from UTEX Culture Collection Source ID: tap-medium, URL: https://utex.org/products/tap-medium
2026-03-13T06:18:53.658341Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000233
2026-03-17T19:45:04.568873+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "TAP Medium" to "tap_medium" for consistent matching
2026-08-06T00:58:02.525354+00:00 curate_organism_culture_type.py SET_ORGANISM_CULTURE_TYPE Inferred isolate: 2 specific strain(s) named, no community signal (#142)
2026-08-30T00:58:31.026248+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 4 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_wc_tap_waris_score35.py RESOLVED_UTEX_WC_TAP_WARIS_SCORE35 UTEX prepares TAP Medium from Beijerinck's Solution, Phosphate Buffer Stock Solution, Hunter's Trace Stock Solution, and Tris Acetate Stock Solution added at source-stated volumes.