volvocacean_3n_medium

CultureMech:000235 algae COMPLEX LIQUID
Original name: Volvocacean-3N Medium

Overview

External ontology
UTEX:volvocacean-3n-medium (source link unavailable) — Volvocacean-3N Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH
6.0
Notes
UTEX prepares Volvocacean-3N Medium at pH 6.0 from a Volvocacean salt base, P-IV Metal Solution, and 200 mL/L prepared Euglena Medium.

Composition

flowchart LR
    CultureMech_000235["volvocacean_3n_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000235 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_KNO3["KNO3"]
    CultureMech_000235 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000235 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3__NH4_2HPO4["(NH4)2HPO4"]
    CultureMech_000235 --> ing3__NH4_2HPO4
    CHEBI_63051["CHEBI:63051"]
    ing3__NH4_2HPO4 -.-> CHEBI_63051
    ing4_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_000235 --> ing4_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing4_CaSO4_x_2_H2O -.-> CHEBI_32583

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
800 ML_PER_L CHEBI:15377
water
 UTEX prepares a 1 L base recipe in dH2O, discards 200 mL of it, and replaces that volume with 200 mL Euglena Medium.
2 KNO3
K.NO3 101.102 g/mol
Baker 3190
0.98 MILLIMOLAR CHEBI:63043
potassium nitrate
 UTEX adds 1 mL/L of a 10 g/100 mL dH2O stock, for 0.98 mM final.
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
Sigma 230391
0.081 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 UTEX adds 1 mL/L of a 2 g/100 mL dH2O stock, for 0.081 mM final.
4 (NH4)2HPO4
2H4N.HO4P 132.056 g/mol
Fisher A686
0.453 MILLIMOLAR CHEBI:63051
diammonium hydrogen phosphate
 UTEX adds 3 mL/L of a 2 g/100 mL dH2O stock, for 0.453 mM final.
5 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
Mallinckrodt 4300
0.35 MILLIMOLAR CHEBI:32583
calcium sulfate dihydrate
 UTEX adds 30 mL/L of a 0.2 g/100 mL dH2O stock, for 0.35 mM final.

Preparation

  1. To approximately 900 mL dH2O, add the listed components except Euglena Medium while stirring continuously.
  2. Adjust the medium to pH 6.0.
  3. Bring the total volume to 1 L with dH2O.
  4. Discard 200 mL of this medium.
  5. Transfer 200 mL of previously prepared Euglena Medium into the flask.
  6. Autoclave the medium.
  7. Store at refrigerator temperature.

Solutions

P-IV Metal Solution

Dose: 6 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings P-IV Metal Solution to 1 L with dH2O.
Na2EDTA x 2 H2O 0.75 G_PER_L UTEX lists 0.75 g/L Na2EDTA x 2 H2O, 2 mM final, and dissolves it fully before the other P-IV components.
FeCl3 x 6 H2O 0.097 G_PER_L UTEX lists 0.097 g/L FeCl3 x 6 H2O, for 0.36 mM final.
MnCl2 x 4 H2O 0.041 G_PER_L UTEX lists 0.041 g/L MnCl2 x 4 H2O, for 0.21 mM final.
ZnCl2 0.005 G_PER_L UTEX lists 0.005 g/L ZnCl2, for 0.037 mM final.
CoCl2 x 6 H2O 0.002 G_PER_L UTEX lists 0.002 g/L CoCl2 x 6 H2O, for 0.0084 mM final.
Na2MoO4 x 2 H2O 0.004 G_PER_L UTEX lists 0.004 g/L Na2MoO4 x 2 H2O, for 0.017 mM final.
UTEX adds 6 mL P-IV Metal Solution per liter.

Euglena Medium

Dose: 200 ML_PER_L

CultureMech:000168

UTEX replaces 200 mL of the base recipe with 200 mL prepared Euglena Medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: volvocacean-3n-medium, URL: https://utex.org/products/volvocacean-3n-medium
2026-03-13T06:18:53.668872Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000235
2026-03-17T19:45:04.577735+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Volvocacean-3N Medium" to "volvocacean_3n_medium" for consistent matching
2026-05-16T09:02:57.093454+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:02.023625+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:32.314928+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 6 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_volvox_p49_score35.py RESOLVED_UTEX_VOLVOX_P49_SCORE35 UTEX prepares Volvocacean-3N Medium at pH 6.0 from a Volvocacean salt base, P-IV Metal Solution, and 200 mL/L prepared Euglena Medium.