volvox_medium

CultureMech:000238 algae COMPLEX LIQUID
Original name: Volvox Medium

Overview

External ontology
UTEX:volvox-medium (source link unavailable) — Volvox Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH
8.0
Notes
UTEX prepares Volvox Medium at pH 8.0 from calcium nitrate, magnesium sulfate, sodium glycerophosphate, potassium chloride, glycylglycine, P-IV Metal Solution, Vitamin B12, and Biotin Vitamin Solution.

Composition

flowchart LR
    CultureMech_000238["volvox_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000238 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000238 --> ing1_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing1_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000238 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_Na2glycerophosphate_x_5_H2O["Na2glycerophosphate x 5 H2O"]
    CultureMech_000238 --> ing3_Na2glycerophosphate_x_5_H2O
    CHEBI_15978["CHEBI:15978"]
    ing3_Na2glycerophosphate_x_5_H2O -.-> CHEBI_15978
    ing4_KCl["KCl"]
    CultureMech_000238 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Glycylglycine["Glycylglycine"]
    CultureMech_000238 --> ing5_Glycylglycine
    CHEBI_17201["CHEBI:17201"]
    ing5_Glycylglycine -.-> CHEBI_17201

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts the 1 L Volvox Medium recipe with approximately 950 mL dH2O.
2 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
0.5 MILLIMOLAR CHEBI:86159
calcium nitrate tetrahydrate
 UTEX adds 1 mL/L of an 11.8 g/100 mL dH2O stock, for 0.5 mM final.
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.16 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 UTEX adds 1 mL/L of a 4 g/100 mL dH2O stock, for 0.16 mM final.
4 Na2glycerophosphate x 5 H2O
C3H9O6P 172.073 g/mol
0.16 MILLIMOLAR CHEBI:15978
sn-glycerol 3-phosphate
 UTEX adds 1 mL/L of a 5 g/100 mL dH2O stock, for 0.16 mM final.
5 KCl
Cl.K 74.551 g/mol
0.67 MILLIMOLAR CHEBI:32588
potassium chloride
 UTEX adds 1 mL/L of a 5 g/100 mL dH2O stock, for 0.67 mM final.
6 Glycylglycine
C4H8N2O3 132.119 g/mol
3.8 MILLIMOLAR CHEBI:17201
glycylglycine
 UTEX adds 10 mL/L of a 5 g/100 mL dH2O stock, for 3.8 mM final.

Preparation

  1. To approximately 950 mL dH2O, add the salts, glycylglycine, and P-IV Metal Solution in order while stirring continuously.
  2. Adjust the medium to pH 8.0.
  3. Bring the total volume to 1 L with dH2O.
  4. Cover and autoclave the medium.
  5. Allow the medium to cool, then add Vitamin B12 and Biotin Vitamin Solution.

Solutions

P-IV Metal Solution

Dose: 6 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings P-IV Metal Solution to 1 L with dH2O.
Na2EDTA x 2 H2O 0.75 G_PER_L UTEX lists 0.75 g/L Na2EDTA x 2 H2O, 2 mM final, and dissolves it fully before the other P-IV components.
FeCl3 x 6 H2O 0.097 G_PER_L UTEX lists 0.097 g/L FeCl3 x 6 H2O, for 0.36 mM final.
MnCl2 x 4 H2O 0.041 G_PER_L UTEX lists 0.041 g/L MnCl2 x 4 H2O, for 0.21 mM final.
ZnCl2 0.005 G_PER_L UTEX lists 0.005 g/L ZnCl2, for 0.037 mM final.
CoCl2 x 6 H2O 0.002 G_PER_L UTEX lists 0.002 g/L CoCl2 x 6 H2O, for 0.0084 mM final.
Na2MoO4 x 2 H2O 0.004 G_PER_L UTEX lists 0.004 g/L Na2MoO4 x 2 H2O, for 0.017 mM final.
UTEX adds 6 mL P-IV Metal Solution per liter.

Vitamin B12

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the Vitamin B12 stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock.
Vitamin B12 0.135 G_PER_L Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock.
UTEX adds 1 mL Vitamin B12 stock per liter.

Biotin Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the biotin stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX biotin stock.
Biotin 0.025 G_PER_L Converted from 0.005 g/200 mL in the UTEX biotin stock.
UTEX adds 1 mL Biotin Vitamin Solution per liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: volvox-medium, URL: https://utex.org/products/volvox-medium
2026-03-13T06:18:53.684075Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000238
2026-03-17T19:45:04.590968+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Volvox Medium" to "volvox_medium" for consistent matching
2026-05-16T09:02:56.161543+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.380411+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:31.047848+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 8 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_organic_score35.py RESOLVED_UTEX_ORGANIC_SCORE35 UTEX prepares Volvox Medium at pH 8.0 from calcium nitrate, magnesium sulfate, sodium glycerophosphate, potassium chloride, glycylglycine, P-IV Metal Solution, Vitamin B12, and Biotin Vitamin Solution.