wc_medium

CultureMech:000239 algae COMPLEX LIQUID
Original name: WC+ Medium

Overview

External ontology
UTEX:wc-plus-medium (source link unavailable) — WC+ Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH
7.8
Notes
UTEX prepares WC+ Medium at pH 7.8 from the WC salt and vitamin base with doubled sodium silicate and added DYV Metal Solution.

Composition

flowchart LR
    CultureMech_000239["wc_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000239 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_Na2SiO3_x_9_H2O["Na2SiO3 x 9 H2O"]
    CultureMech_000239 --> ing1_Na2SiO3_x_9_H2O
    CHEBI_132108["CHEBI:132108"]
    ing1_Na2SiO3_x_9_H2O -.-> CHEBI_132108
    ing2_NaNO3["NaNO3"]
    CultureMech_000239 --> ing2_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing2_NaNO3 -.-> CHEBI_63005
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000239 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000239 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_NaHCO3["NaHCO3"]
    CultureMech_000239 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_K2HPO4["K2HPO4"]
    CultureMech_000239 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_H3BO3["H3BO3"]
    CultureMech_000239 --> ing7_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing7_H3BO3 -.-> CHEBI_33118

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts the 1 L WC recipe with approximately 900 mL dH2O.
2 Na2SiO3 x 9 H2O
9H2O.2Na.O3Si 284.198 g/mol
Sigma 307815
0.2 MILLIMOLAR CHEBI:132108
sodium silicate nonahydrate
 UTEX adds 2 mL/L of a 28.42 g/L stock, for 0.2 mM final.
3 NaNO3
NO3.Na 84.994 g/mol
Fisher BP360-500
1 MILLIMOLAR CHEBI:63005
sodium nitrate
 UTEX adds 1 mL/L of an 85.1 g/L stock, for 1 mM final.
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
Sigma C-3881
0.25 MILLIMOLAR CHEBI:86158
calcium chloride dihydrate
 UTEX adds 1 mL/L of a 36.76 g/L stock, for 0.25 mM final.
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
Sigma 230391
0.15 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 UTEX adds 1 mL/L of a 36.97 g/L stock, for 0.15 mM final.
6 NaHCO3
CHO3.Na 84.006 g/mol
Fisher S 233
0.15 MILLIMOLAR CHEBI:32139
sodium hydrogencarbonate
 UTEX adds 1 mL/L of a 12.6 g/L stock, for 0.15 mM final.
7 K2HPO4
HO4P.2K 174.174 g/mol
Sigma P 3786
0.05 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
 UTEX adds 1 mL/L of an 8.71 g/L stock, for 0.05 mM final.
8 H3BO3
H3BO3 61.833 g/mol
Baker 0084
0.39 MILLIMOLAR CHEBI:33118
boric acid
 UTEX adds 1 mL/L of a 24 g/L stock, for 0.39 mM final.

Preparation

  1. To approximately 900 mL dH2O, add WC Trace Elements Solution, DYV Metal Solution and each non-vitamin WC component in the UTEX order.
  2. Adjust the medium to pH 7.8.
  3. Bring total volume to 1 L with dH2O.
  4. Filter sterilize the medium.
  5. Aseptically add sterile Vitamin B12, Thiamine Vitamin Solution, and Biotin Vitamin Solution.
  6. Store at refrigerator temperature.

Solutions

WC Trace Elements Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX starts WC Trace Elements Solution with approximately 950 mL dH2O.
Na2EDTA x 2 H2O 4.36 G_PER_L UTEX lists 4.36 g/L Na2EDTA x 2 H2O in WC Trace Elements Solution, for 11.7 uM final in WC Medium.
FeCl3 x 6 H2O 3.15 G_PER_L UTEX lists 3.15 g/L FeCl3 x 6 H2O in WC Trace Elements Solution, for 11.7 uM final in WC Medium.
CuSO4 x 5 H2O 1 ML_PER_L UTEX adds 1 mL/L of a 2.5 g/L CuSO4 x 5 H2O substock to WC Trace Elements Solution, for 10 nM final in WC Medium.
ZnSO4 x 7 H2O 1 ML_PER_L UTEX adds 1 mL/L of a 22 g/L ZnSO4 x 7 H2O substock to WC Trace Elements Solution, for 76.5 nM final in WC Medium.
CoCl2 x 6 H2O 1 ML_PER_L UTEX adds 1 mL/L of a 10 g/L CoCl2 x 6 H2O substock to WC Trace Elements Solution, for 42 nM final in WC Medium.
MnCl2 x 4 H2O 1 ML_PER_L UTEX adds 1 mL/L of a 180 g/L MnCl2 x 4 H2O substock to WC Trace Elements Solution, for 910 nM final in WC Medium.
Na2MoO4 x 2 H2O 1 ML_PER_L UTEX adds 1 mL/L of a 6.3 g/L Na2MoO4 x 2 H2O substock to WC Trace Elements Solution, for 26 nM final in WC Medium.
Na3VO4 1 ML_PER_L UTEX adds 1 mL/L of an 18 g/L Na3VO4 substock to WC Trace Elements Solution, for 98 nM final in WC Medium.
UTEX adds 1 mL WC Trace Elements Solution per liter.

DYV Metal Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings DYV Metal Solution to 1 L with dH2O.
Na3VO4 0.002 G_PER_L UTEX lists 0.002 g/L Na3VO4.
H2SeO3 0.004 G_PER_L UTEX lists 0.004 g/L H2SeO3.
UTEX adds 1 mL DYV Metal Solution per liter.

Vitamin B12

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the Vitamin B12 stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock.
Vitamin B12 0.135 G_PER_L Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock.
UTEX adds 1 mL Vitamin B12 stock per liter.

Thiamine Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the thiamine stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX thiamine stock.
Thiamine 0.335 G_PER_L Converted from 0.067 g/200 mL in the UTEX thiamine stock.
UTEX adds 1 mL Thiamine Vitamin Solution per liter.

Biotin Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the biotin stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX biotin stock.
Biotin 0.025 G_PER_L Converted from 0.005 g/200 mL in the UTEX biotin stock.
UTEX adds 1 mL Biotin Vitamin Solution per liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: wc-plus-medium, URL: https://utex.org/products/wc-plus-medium
2026-03-13T06:18:53.691350Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000239
2026-03-17T19:45:04.596600+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "WC+ Medium" to "wc_medium" for consistent matching
2026-05-16T09:02:56.167653+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.387476+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:31.055757+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 12 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_wc_tap_waris_score35.py RESOLVED_UTEX_WC_TAP_WARIS_SCORE35 UTEX prepares WC+ Medium at pH 7.8 from the WC salt and vitamin base with doubled sodium silicate and added DYV Metal Solution.