methermicoccus_methanogen_medium

Overview

External ontology

mediadive.medium:1084 (source link unavailable) — METHERMICOCCUS (METHANOGEN) MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 6.0, 'max': 6.5}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1084.pdf

Composition

flowchart LR
    CultureMech_000246["methermicoccus_methanogen_medium"]
    ing0_KCl["KCl"]
    CultureMech_000246 --> ing0_KCl
    CHEBI_32588["CHEBI:32588"]
    ing0_KCl -.-> CHEBI_32588
    ing1_NH4Cl["NH4Cl"]
    CultureMech_000246 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000246 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_NaCl["NaCl"]
    CultureMech_000246 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000246 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_000246 --> ing5_Yeast_extract
    ing6_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000246 --> ing6_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Sodium_resazurin -.-> CHEBI_8806
    ing7_Na2CO3["Na2CO3"]
    CultureMech_000246 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_Methanol["Methanol"]
    CultureMech_000246 --> ing8_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing8_Methanol -.-> CHEBI_17790
    ing9_2_Mercaptoethanesulfonate["2-Mercaptoethanesulfonate"]
    CultureMech_000246 --> ing9_2_Mercaptoethanesulfonate
    CHEBI_17905["CHEBI:17905"]
    ing9_2_Mercaptoethanesulfonate -.-> CHEBI_17905
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000246 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000246 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Sludge["Sludge"]
    CultureMech_000246 --> ing12_Sludge

Ingredients (13)

Preparation

1. Dissolve ingredients (except carbonate, sludge fluid, methanol, coenzyme M, cysteine and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add sludge fluid, methanol (50% v/v solution), coenzyme M (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 6.0 - 6.5, if necessary.
2. Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37°C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at 8-12°C in the dark.

Target organisms

No target organisms are recorded.

Provenance