medium_for_halophilic_archaea

CultureMech:000249 archaea COMPLEX SOLID_AGAR
Original name: MEDIUM FOR HALOPHILIC ARCHAEA

Overview

External ontology
mediadive.medium:1184 (source link unavailable) — MEDIUM FOR HALOPHILIC ARCHAEA
Applications
  • Microbial cultivation
pH range
{'min': 6.5, 'max': 7.0}
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1184.pdf

Composition

flowchart LR
    CultureMech_000249["medium_for_halophilic_archaea"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_000249 --> ing0_Yeast_extract
    ing1_KCl["KCl"]
    CultureMech_000249 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000249 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000249 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000249 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NaHCO3["NaHCO3"]
    CultureMech_000249 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_NaBr["NaBr"]
    CultureMech_000249 --> ing6_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing6_NaBr -.-> CHEBI_63004
    ing7_NaCl["NaCl"]
    CultureMech_000249 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_Agar["Agar"]
    CultureMech_000249 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Yeast extract 5 G_PER_L
2 KCl
Cl.K 74.551 g/mol
5 G_PER_L CHEBI:32588
potassium chloride
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
32.5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
50.8 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.8 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 NaHCO3
CHO3.Na 84.006 g/mol
0.16 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 NaBr
Br.Na 102.894 g/mol
0.6 G_PER_L CHEBI:63004
sodium bromide
8 NaCl
Cl.Na 58.443 g/mol
195 G_PER_L CHEBI:26710
sodium chloride
9 Agar 20 G_PER_L CHEBI:2509
agar
 (for solid medium)

Preparation

  1. The medium may be solidified by adding 20.0 g/l agar.
  2. For solid medium add the NaCl after autoclaving.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.615538Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1184
2026-03-13T06:18:53.767246Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000249
2026-03-17T17:06:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:04.673973+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM FOR HALOPHILIC ARCHAEA" to "medium_for_halophilic_archaea" for consistent matching
2026-05-16T09:02:57.370319+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS category->lowercase=1
2026-05-20T06:00:46.081474+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.5-7' -> {'min': 6.5, 'max': 7.0}
2026-06-05T07:40:14.647883+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.219950+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:32:40.086487+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.