Overview
- External ontology
- mediadive.medium:1400 (source link unavailable) — SALINIBACTER HALOPHILIC MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1400.pdf
Composition
flowchart LR
CultureMech_000253["salinibacter_halophilic_medium"]
ing0_NaCl["NaCl"]
CultureMech_000253 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000253 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_000253 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Yeast_extract["Yeast extract"]
CultureMech_000253 --> ing3_Yeast_extract
ing4_Bacto_peptone["Bacto peptone"]
CultureMech_000253 --> ing4_Bacto_peptone
ing5_Casamino_acids["Casamino acids"]
CultureMech_000253 --> ing5_Casamino_acids
ing6_Glucose["Glucose"]
CultureMech_000253 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Starch["Starch"]
CultureMech_000253 --> ing7_Starch
CHEBI_28017["CHEBI:28017"]
ing7_Starch -.-> CHEBI_28017
ing8_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_000253 --> ing8_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing8_Sodium_pyruvate -.-> CHEBI_50144
ing9_Agar["Agar"]
CultureMech_000253 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 | Yeast extract | 0.5 G_PER_L | ||
| 5 | Bacto peptone | 0.5 G_PER_L | ||
| 6 | Casamino acids | 0.5 G_PER_L | ||
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
0.772 G_PER_L |
CHEBI:17234
glucose
|
|
| 8 | Starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
|
| 9 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 10 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Adjust to pH 7.0 and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.313956Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1400 |
| 2026-01-28T19:48:44.607019Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:53.791429Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000253 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:04.700024+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SALINIBACTER HALOPHILIC MEDIUM" to "salinibacter_halophilic_medium" for consistent matching |
| 2026-05-16T09:02:57.784343+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-05T07:40:15.082195+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:27.138112+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:42.378194+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |