Overview
- External ontology
- mediadive.medium:1433 (source link unavailable) — ACIDO-THERMOPHILE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1433.pdf Commercial Product: MacConkey Agar Supplier: Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) Catalog: BD 212123, Sigma 05465 Source: https://microbenotes.com/macconkey-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_000256["acido_thermophile_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_000256 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_000256 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Na2HPO4_x_7_H2O["Na2HPO4 x 7 H2O"]
CultureMech_000256 --> ing2_Na2HPO4_x_7_H2O
CHEBI_34683["CHEBI:34683"]
ing2_Na2HPO4_x_7_H2O -.-> CHEBI_34683
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000256 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000256 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Yeast_extract["Yeast extract"]
CultureMech_000256 --> ing5_Yeast_extract
ing6_Peptone["Peptone"]
CultureMech_000256 --> ing6_Peptone
ing7_Proteose_peptone["Proteose peptone"]
CultureMech_000256 --> ing7_Proteose_peptone
ing8_Lactose_monohydrate["Lactose monohydrate"]
CultureMech_000256 --> ing8_Lactose_monohydrate
CHEBI_17716["CHEBI:17716"]
ing8_Lactose_monohydrate -.-> CHEBI_17716
ing9_Bile_salts["Bile salts"]
CultureMech_000256 --> ing9_Bile_salts
CHEBI_22868["CHEBI:22868"]
ing9_Bile_salts -.-> CHEBI_22868
ing10_Sodium_chloride["Sodium chloride"]
CultureMech_000256 --> ing10_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing10_Sodium_chloride -.-> CHEBI_26710
ing11_Neutral_red["Neutral red"]
CultureMech_000256 --> ing11_Neutral_red
CHEBI_86370["CHEBI:86370"]
ing11_Neutral_red -.-> CHEBI_86370
ing12_Crystal_violet["Crystal violet"]
CultureMech_000256 --> ing12_Crystal_violet
CHEBI_41688["CHEBI:41688"]
ing12_Crystal_violet -.-> CHEBI_41688
ing13_Agar["Agar"]
CultureMech_000256 --> ing13_Agar
CHEBI_2509["CHEBI:2509"]
ing13_Agar -.-> CHEBI_2509
ing14_Cellobiose["Cellobiose"]
CultureMech_000256 --> ing14_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing14_Cellobiose -.-> CHEBI_17057
ing15_Agar["Agar"]
CultureMech_000256 --> ing15_Agar
ing15_Agar -.-> CHEBI_2509
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
Na2HPO4 x 7 H2O
HO4P.2Na 141.958 g/mol
|
0.1 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 | Yeast extract | 1 G_PER_L | ||
| 7 |
Peptone
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
17.0 G_PER_L |
MICRO:0000178 (source link unavailable)
peptone
|
Pancreatic digest of gelatin, provides nitrogen and amino acids; Constituent of MacConkey Agar commercial product |
| 8 |
Proteose peptone
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
3.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Meat and casein digest, provides additional nutrients; Constituent of MacConkey Agar commercial product |
| 9 |
Lactose monohydrate
C12H22O11 342.297 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
10.0 G_PER_L |
CHEBI:17716
lactose
|
Fermentable carbohydrate for differentiation. Fermentation produces acid causing pH drop and color change; Constituent of MacConkey Agar commercial product |
| 10 |
Bile salts
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
1.5 G_PER_L |
CHEBI:22868
bile salt
|
Selective agent - inhibits gram-positive bacteria; Constituent of MacConkey Agar commercial product |
| 11 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of MacConkey Agar commercial product |
| 12 |
Neutral red
C15H16N4.HCl 288.782 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
0.03 G_PER_L |
CHEBI:86370
neutral red
|
pH indicator - turns red/pink below pH 6.8, absorbed by colonies of lactose fermenters; Constituent of MacConkey Agar commercial product |
| 13 |
Crystal violet
C25H30N3.Cl 407.989 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
0.001 G_PER_L |
CHEBI:41688
crystal violet
|
Selective agent - inhibits gram-positive bacteria; Constituent of MacConkey Agar commercial product |
| 14 |
Agar
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
13.5 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent; Constituent of MacConkey Agar commercial product |
| 15 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.5 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 16 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust to pH 5.2 with H3PO4 prior to addition of carbon sources.
- Autoclave at 121°C for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.405871Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1433 |
| 2026-03-13T06:18:53.820455Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000256 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:04.732610+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDO-THERMOPHILE MEDIUM" to "acido_thermophile_medium" for consistent matching |
| 2026-05-16T09:02:57.207028+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-05-17T07:06:05.053686+00:00 | cleanup_residual_errors.py | CLEANUP_RESIDUAL_ERRORS | R01_flatten_synonym_text=2 |
| 2026-06-05T07:40:14.471036+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.152852+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-07-29T02:09:00.657591Z | domain-growth-evidence | ANNOTATED | Moved archaea/ -> bacterial/ and restamped category on growth evidence: kg-microbe records only bacterial taxa growing in this medium (Acidothermus cellulolyticus). The name carries a physiology (halophile/thermophile) that spans both domains, so the previous archaea/ placement was unsubstantiated (#138). |