pediococcus_halophilus_medium

CultureMech:000258 bacterial COMPLEX LIQUID
Original name: PEDIOCOCCUS HALOPHILUS MEDIUM

Overview

External ontology
mediadive.medium:227 (source link unavailable) — PEDIOCOCCUS HALOPHILUS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.2, 'max': 6.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium227.pdf

Composition

flowchart LR
    CultureMech_000258["pediococcus_halophilus_medium"]
    ing0_Casein_peptone["Casein peptone"]
    CultureMech_000258 --> ing0_Casein_peptone
    ing1_Meat_extract["Meat extract"]
    CultureMech_000258 --> ing1_Meat_extract
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_000258 --> ing2_Yeast_extract
    ing3_Glucose["Glucose"]
    CultureMech_000258 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Tween_80["Tween 80"]
    CultureMech_000258 --> ing4_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing4_Tween_80 -.-> CHEBI_53426
    ing5_K2HPO4["K2HPO4"]
    CultureMech_000258 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Na_acetate["Na-acetate"]
    CultureMech_000258 --> ing6_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Na_acetate -.-> CHEBI_32954
    ing7__NH4_3_citrate["(NH4)3 citrate"]
    CultureMech_000258 --> ing7__NH4_3_citrate
    CHEBI_63037["CHEBI:63037"]
    ing7__NH4_3_citrate -.-> CHEBI_63037
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000258 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000258 --> ing9_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing9_MnSO4_x_H2O -.-> CHEBI_86364
    ing10_NaCl["NaCl"]
    CultureMech_000258 --> ing10_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing10_NaCl -.-> CHEBI_26710

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Casein peptone 10 G_PER_L
2 Meat extract 10 G_PER_L
3 Yeast extract 5 G_PER_L
4 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
5 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1 G_PER_L CHEBI:53426
polysorbate 80
6 K2HPO4
HO4P.2K 174.174 g/mol
2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
7 Na-acetate
C2H3O2.Na 82.034 g/mol
5 G_PER_L CHEBI:32954
sodium acetate
8 (NH4)3 citrate
C6H5O7.3H4N 243.216 g/mol
2 G_PER_L CHEBI:63037
triammonium citrate
9 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
10 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.05 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
11 NaCl
Cl.Na 58.443 g/mol
65 G_PER_L CHEBI:26710
sodium chloride

Preparation

  1. Adjust pH to 6.2 - 6.5.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.554458Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 227
2026-03-13T06:18:53.830708Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000258
2026-03-17T17:06:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:04.744256+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PEDIOCOCCUS HALOPHILUS MEDIUM" to "pediococcus_halophilus_medium" for consistent matching
2026-05-16T09:02:57.746473+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS category->lowercase=1
2026-05-20T06:00:46.392812+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.2-6.5' -> {'min': 6.2, 'max': 6.5}
2026-06-05T07:40:15.041016+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.362937+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.