Overview
- External ontology
- mediadive.medium:625 (source link unavailable) — HP 101 HALOPHILE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium625.pdf
Composition
flowchart LR
CultureMech_000275["hp_101_halophile_medium"]
ing0_Peptone["Peptone"]
CultureMech_000275 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_000275 --> ing1_Yeast_extract
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000275 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NaNO3["NaNO3"]
CultureMech_000275 --> ing3_NaNO3
CHEBI_63005["CHEBI:63005"]
ing3_NaNO3 -.-> CHEBI_63005
ing4_NaCl["NaCl"]
CultureMech_000275 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Agar["Agar"]
CultureMech_000275 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 10 G_PER_L | ||
| 2 | Yeast extract | 1 G_PER_L | ||
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
4.3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
NaNO3
NO3.Na 84.994 g/mol
|
2 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
100 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.769418Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 625 |
| 2026-03-13T06:18:54.034852Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000275 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:04.966233+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HP 101 HALOPHILE MEDIUM" to "hp_101_halophile_medium" for consistent matching |
| 2026-05-16T09:02:57.320095+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-05T07:40:14.593261+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T12:21:30.028294+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |