halophile_medium

CultureMech:000276 archaea COMPLEX LIQUID
Original name: HALOPHILE MEDIUM

Overview

External ontology
mediadive.medium:652 (source link unavailable) — HALOPHILE MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.5, 'max': 8.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium652.pdf

Composition

flowchart LR
    CultureMech_000276["halophile_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000276 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_000276 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000276 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000276 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000276 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_000276 --> ing5_Yeast_extract
    ing6_Peptone["Peptone"]
    CultureMech_000276 --> ing6_Peptone
    ing7_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_000276 --> ing7_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing7_Fe_III__citrate -.-> CHEBI_144421
    ing8_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000276 --> ing8_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing8_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000276 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000276 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000276 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_Na2MoO4_x_H2O["Na2MoO4 x H2O"]
    CultureMech_000276 --> ing12_Na2MoO4_x_H2O
    CHEBI_75215["CHEBI:75215"]
    ing12_Na2MoO4_x_H2O -.-> CHEBI_75215

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
5 G_PER_L CHEBI:32588
potassium chloride
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 NH4Cl
Cl.H4N 53.492 g/mol
5 G_PER_L CHEBI:31206
ammonium chloride
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 Yeast extract 5 G_PER_L
7 Peptone 5 G_PER_L
8 Fe(III) citrate
C6H5FeO7 244.944 g/mol
0.05 G_PER_L CHEBI:144421
iron(III) citrate
9 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.001 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.22 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.01 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.18 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 Na2MoO4 x H2O
MoO4.2Na 205.916 g/mol
0.0063 G_PER_L CHEBI:75215
sodium molybdate (anhydrous)

Preparation

  1. Adjust pH to 7.5-8.0. Boil the medium for few minutes. Fill in tubes with rubber stoppers under nitrogen gas. Sterilize at 121°C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.845343Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 652
2026-03-13T06:18:54.040395Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000276
2026-03-17T17:06:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:04.972123+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOPHILE MEDIUM" to "halophile_medium" for consistent matching
2026-05-16T09:02:57.276376+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS category->lowercase=1
2026-05-20T06:00:46.007835+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.5-8' -> {'min': 7.5, 'max': 8.0}
2026-06-05T07:40:14.546070+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.182574+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.