Overview
- External ontology
- mediadive.medium:674 (source link unavailable) — MEDIUM FOR ACIDURIC, THERMOPHILIC BACILLUS STRAINS
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium674.pdf
Composition
flowchart LR
CultureMech_000277["medium_for_aciduric_thermophilic_bacillus_strains"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_000277 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000277 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000277 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_000277 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_Yeast_extract["Yeast extract"]
CultureMech_000277 --> ing4_Yeast_extract
ing5_Tryptone["Tryptone"]
CultureMech_000277 --> ing5_Tryptone
ing6_Glucose["Glucose"]
CultureMech_000277 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Starch["Starch"]
CultureMech_000277 --> ing7_Starch
CHEBI_28017["CHEBI:28017"]
ing7_Starch -.-> CHEBI_28017
ing8_Agar["Agar"]
CultureMech_000277 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.4 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 | Yeast extract | 2 G_PER_L | ||
| 6 | Tryptone | 2 G_PER_L | ||
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
2 G_PER_L |
CHEBI:17234
glucose
|
|
| 8 | Starch | 2 G_PER_L |
CHEBI:28017
starch
|
|
| 9 | Agar | 40 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Solutions A and B are sterilized separately. After cooling to approximately 45°C solution A is poured into solution B. Mix thoroughly.
- Adjust pH to 4.3.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.903230Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 674 |
| 2026-03-13T06:18:54.046135Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000277 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:04.978260+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM FOR ACIDURIC, THERMOPHILIC BACILLUS STRAINS" to "medium_for_aciduric_thermophilic_bacillus_strains" for consistent matching |
| 2026-05-16T09:02:57.355210+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-05T07:40:14.630781+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.213657+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |