desulfocella_halophila_medium

CultureMech:000283 bacterial COMPLEX LIQUID
Original name: DESULFOCELLA HALOPHILA MEDIUM

Overview

External ontology
mediadive.medium:815a (source link unavailable) — DESULFOCELLA HALOPHILA MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium815a.pdf

Composition

flowchart LR
    CultureMech_000283["desulfocella_halophila_medium"]
    ing0_Na2SO4["Na2SO4"]
    CultureMech_000283 --> ing0_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing0_Na2SO4 -.-> CHEBI_32149
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000283 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_NaCl["NaCl"]
    CultureMech_000283 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KCl["KCl"]
    CultureMech_000283 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NH4Cl["NH4Cl"]
    CultureMech_000283 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_KH2PO4["KH2PO4"]
    CultureMech_000283 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000283 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_000283 --> ing7_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing7_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing8_MOPS["MOPS"]
    CultureMech_000283 --> ing8_MOPS
    CHEBI_39074["CHEBI:39074"]
    ing8_MOPS -.-> CHEBI_39074
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_000283 --> ing9_Yeast_extract
    ing10_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000283 --> ing10_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Sodium_resazurin -.-> CHEBI_8806
    ing11_Na_pyruvate["Na-pyruvate"]
    CultureMech_000283 --> ing11_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing11_Na_pyruvate -.-> CHEBI_50144
    ing12_Na2CO3["Na2CO3"]
    CultureMech_000283 --> ing12_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing12_Na2CO3 -.-> CHEBI_29377
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000283 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_000283 --> ing14_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing14_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000283 --> ing15_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing16_ZnCl2["ZnCl2"]
    CultureMech_000283 --> ing16_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing16_ZnCl2 -.-> CHEBI_49976
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000283 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_H3BO3["H3BO3"]
    CultureMech_000283 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000283 --> ing19_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000283 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000283 --> ing21_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000283 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 Na2SO4
2Na.O4S 142.043 g/mol
3 G_PER_L CHEBI:32149
sodium sulfate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
6 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 NaCl
Cl.Na 58.443 g/mol
40 G_PER_L CHEBI:26710
sodium chloride
4 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
5 NH4Cl
Cl.H4N 53.492 g/mol
0.3 G_PER_L CHEBI:31206
ammonium chloride
6 KH2PO4
H2O4P.K 136.084 g/mol
0.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
8 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.1 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
9 MOPS 3 G_PER_L CHEBI:39074
MOPS
10 Yeast extract 1 G_PER_L
11 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
12 Na-pyruvate
C3H3O3.Na 110.044 g/mol
2.5 G_PER_L CHEBI:50144
sodium pyruvate
13 Na2CO3
CO3.2Na 105.988 g/mol
0.5 G_PER_L CHEBI:29377
sodium carbonate
14 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.1 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
15 Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
5.2 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
16 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
17 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
18 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
19 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
20 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
21 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
22 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Dissolve ingredients except pyruvate, bicarbonate and sulfide, adjust pH to 6.0 with NaOH and sparge medium for 30 - 45 min with 100% N2 gas to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add pyruvate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Final medium pH should be 7.2.
  2. Note: Prior to inoculation 10-20 mg/l sodium dithionite (added from a 5% w/v solution freshly prepared under N2 and filter-sterilized) can be added to the medium to stimulate growth at the beginning.
  3. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.349702Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 815a
2026-03-13T06:18:54.100366Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000283
2026-03-17T17:06:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.038733+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFOCELLA HALOPHILA MEDIUM" to "desulfocella_halophila_medium" for consistent matching
2026-05-16T09:02:57.233188+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS category->lowercase=1
2026-06-05T07:40:14.499653+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.163699+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.