Overview
- External ontology
- mediadive.medium:J1027 (source link unavailable) — METHANOGENS SALINE WATER MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1027
Composition
flowchart LR
CultureMech_000286["methanogens_saline_water_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_000286 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_000286 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000286 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_K2HPO4["K2HPO4"]
CultureMech_000286 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000286 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_NaCl["NaCl"]
CultureMech_000286 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_NaHCO3["NaHCO3"]
CultureMech_000286 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Resazurin["Resazurin"]
CultureMech_000286 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Trimethylamine["Trimethylamine"]
CultureMech_000286 --> ing8_Trimethylamine
CHEBI_18139["CHEBI:18139"]
ing8_Trimethylamine -.-> CHEBI_18139
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_000286 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_000286 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_000286 --> ing11_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000286 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000286 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_ZnCl2["ZnCl2"]
CultureMech_000286 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000286 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
ing16_H3BO3["H3BO3"]
CultureMech_000286 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000286 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000286 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_AlCl3["AlCl3"]
CultureMech_000286 --> ing19_AlCl3
CHEBI_30114["CHEBI:30114"]
ing19_AlCl3 -.-> CHEBI_30114
ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_000286 --> ing20_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_000286 --> ing21_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing22_Biotin["Biotin"]
CultureMech_000286 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_Folic_acid["Folic acid"]
CultureMech_000286 --> ing23_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing23_Folic_acid -.-> CHEBI_27470
ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000286 --> ing24_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing25_Thiamine_HCl["Thiamine HCl"]
CultureMech_000286 --> ing25_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing25_Thiamine_HCl -.-> CHEBI_49105
ing26_Riboflavin["Riboflavin"]
CultureMech_000286 --> ing26_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing26_Riboflavin -.-> CHEBI_17015
ing27_Nicotinic_acid["Nicotinic acid"]
CultureMech_000286 --> ing27_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinic_acid -.-> CHEBI_15940
ing28_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_000286 --> ing28_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing28_DL_Calcium_pantothenate -.-> CHEBI_31345
ing29_Vitamin_B12["Vitamin B12"]
CultureMech_000286 --> ing29_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing29_Vitamin_B12 -.-> CHEBI_176843
CultureMech_000286 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.522749 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.135528 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3.38819 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.290416 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.145208 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
4.84027 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.4395 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000968054 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Trimethylamine
C3H9N 59.112 g/mol
|
10 G_PER_L |
CHEBI:18139
trimethylamine
|
|
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 12 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.99 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.198 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.13 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.136 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.0017 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.0062 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.024 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.013 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
AlCl3
AlCl3 133.341 g/mol
|
0.013 G_PER_L |
CHEBI:30114
aluminium trichloride
|
|
| 21 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0017 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 22 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0033 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0049 G_PER_L |
CHEBI:15956
biotin
|
|
| 24 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.0088 G_PER_L |
CHEBI:27470
folic acid
|
|
| 25 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.0041 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 26 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.0067 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 27 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.0075 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 28 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.0025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 29 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.0095 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 30 | Vitamin B12 | 0.0271 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 31 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.0027 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 32 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.0041 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components thoroughly and distribute the medium into culture vessels (e.g., 20 ml medium in 100 ml serum bottles or 5.0 ml medium in Hungate tubes) under a stream of H2-CO2 (80:20, v/v), seal with butyl rubber stoppers and autoclave. Allow to stand the autoclaved medium overnight. Add per liter the following solution from a sterile anaerobic stock (filter-sterilized and stored under a N2 atmosphere):
- Prior to inoculation, add per liter the following solutions aseptically and anaerobically (autoclaved and stored under a N2 atmosphere):
- Comments: For strain JCM 19935, replace trimethylamine with yeast extract (final 0.1 g/L) and pressurize to 100 kPa H2-CO2 (80:20, v/v). For strain JCM 19936, prepare the medium without trimethylamine. Pressurize inoculated culture vessels to 100 kPa H2-CO2 (80:20, v/v).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.363544Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1027 |
| 2026-03-13T06:18:54.133495Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000286 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.075671+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOGENS SALINE WATER MEDIUM" to "methanogens_saline_water_medium" for consistent matching |
| 2026-05-16T09:02:57.486456+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-05T07:40:14.771178+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.260164+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |