hp_101_halophile_medium

CultureMech:000299 bacterial COMPLEX SOLID_AGAR
Original name: HP 101 HALOPHILE MEDIUM

Overview

External ontology
mediadive.medium:J464 (source link unavailable) — HP 101 HALOPHILE MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=464

Composition

flowchart LR
    CultureMech_000299["hp_101_halophile_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_000299 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000299 --> ing1_Yeast_extract
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000299 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NaNO3["NaNO3"]
    CultureMech_000299 --> ing3_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing3_NaNO3 -.-> CHEBI_63005
    ing4_NaCl["NaCl"]
    CultureMech_000299 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Agar["Agar"]
    CultureMech_000299 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Peptone 10 G_PER_L
2 Yeast extract 1 G_PER_L
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
4.3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 NaNO3
NO3.Na 84.994 g/mol
2 G_PER_L CHEBI:63005
sodium nitrate
5 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
6 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.2.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.681106Z mediadive-import Imported from MediaDive Source: JCM, ID: J464
2026-03-13T06:18:54.277917Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000299
2026-03-17T17:06:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.237230+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HP 101 HALOPHILE MEDIUM" to "hp_101_halophile_medium" for consistent matching
2026-05-16T09:02:57.198320+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS category->lowercase=1
2026-06-05T07:40:14.460888+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-07-29T02:09:00.657591Z domain-growth-evidence ANNOTATED Moved archaea/ -> bacterial/ and restamped category on growth evidence: kg-microbe records only bacterial taxa growing in this medium (Halobacillus kuroshimensis DSM 18393, Halobacillus profundi, Virgibacillus salarius). The name carries a physiology (halophile/thermophile) that spans both domains, so the previous archaea/ placement was unsubstantiated (#138).
2026-10-07T14:33:27.670564+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.