modified_balchs_methanogen_medium_1_b

CultureMech:000304 archaea COMPLEX LIQUID
Original name: MODIFIED BALCH'S METHANOGEN MEDIUM 1/B

Overview

External ontology
mediadive.medium:J948 (source link unavailable) — MODIFIED BALCH'S METHANOGEN MEDIUM 1/B
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=948

Composition

flowchart LR
    CultureMech_000304["modified_balchs_methanogen_medium_1_b"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000304 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000304 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_000304 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_000304 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000304 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000304 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000304 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_000304 --> ing7_Yeast_extract
    ing8_Sodium_acetate["Sodium acetate"]
    CultureMech_000304 --> ing8_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing8_Sodium_acetate -.-> CHEBI_32954
    ing9_Sodium_formate["Sodium formate"]
    CultureMech_000304 --> ing9_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing9_Sodium_formate -.-> CHEBI_62965
    ing10_NaHCO3["NaHCO3"]
    CultureMech_000304 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Resazurin["Resazurin"]
    CultureMech_000304 --> ing11_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing11_Resazurin -.-> CHEBI_8806
    ing12_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000304 --> ing12_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing12_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000304 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000304 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000304 --> ing15_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing16_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_000304 --> ing16_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing16_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_000304 --> ing17_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000304 --> ing18_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000304 --> ing19_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing20_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_000304 --> ing20_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing20_AlK_SO4_2 -.-> CHEBI_86463
    ing21_H3BO3["H3BO3"]
    CultureMech_000304 --> ing21_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing21_H3BO3 -.-> CHEBI_33118
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000304 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing23_Biotin["Biotin"]
    CultureMech_000304 --> ing23_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotin -.-> CHEBI_15956
    ing24_Folic_acid["Folic acid"]
    CultureMech_000304 --> ing24_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing24_Folic_acid -.-> CHEBI_27470
    ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000304 --> ing25_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing26_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000304 --> ing26_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing26_Thiamine_HCl -.-> CHEBI_49105
    ing27_Riboflavin["Riboflavin"]
    CultureMech_000304 --> ing27_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing27_Riboflavin -.-> CHEBI_17015
    ing28_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000304 --> ing28_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing28_Nicotinic_acid -.-> CHEBI_15940
    ing29_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000304 --> ing29_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing29_Calcium_pantothenate -.-> CHEBI_31345
    CultureMech_000304 --> more["...3 more"]

Ingredients (33)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.288462 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.288462 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.288462 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
1.5769229999999999 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 0.576923, 1.0]
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.125 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.10192308 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.00192308, 0.1]
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.10769231 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.00769231, 0.1]
8 Yeast extract 0.192308 G_PER_L
9 Sodium acetate
C2H3O2.Na 82.034 g/mol
2.40385 G_PER_L CHEBI:32954
sodium acetate
10 Sodium formate
CHO2.Na 68.007 g/mol
2.40385 G_PER_L CHEBI:62965
sodium formate
11 NaHCO3
CHO3.Na 84.006 g/mol
4.80769 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 Resazurin
C12H7NO4 229.191 g/mol
0.000961538 G_PER_L CHEBI:8806
Resazurin
13 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
10 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
14 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
16 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
17 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
18 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
19 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
20 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
21 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
22 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
24 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
25 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
26 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
27 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
28 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
29 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
30 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
31 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
32 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
33 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix ingredients, except NaHCO3, and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20, v/v), and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., serum bottles, Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Allow to stand the autoclaved medium overnight. Prior to inoculation, add per liter the following solutions aseptically and anaerobically (autoclaved and stored under a N2 atmosphere):
  2. Pressurize the inoculated culture vessels to 200 kPa H2-CO2 (80:20, v/v).
  3. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.098156Z mediadive-import Imported from MediaDive Source: JCM, ID: J948
2026-03-13T03:09:47.195044Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:18:54.322533Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000304
2026-03-17T17:06:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.285362+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED BALCH'S METHANOGEN MEDIUM 1/B" to "modified_balchs_methanogen_medium_1_b" for consistent matching
2026-05-16T09:02:57.698802+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS category->lowercase=1
2026-06-05T07:40:14.990539+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.342149+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.